A kind of Ehrigia spinosa for synthesizing swainsonine and its preparation method and application

A technology of swainsonin and Oxytropis, applied in the field of microorganisms, can solve the problems of lack of swainsonin sources and high price

Inactive Publication Date: 2011-12-14
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Swainsonine has good prospects for developing new anti-tumor drugs, but the source of swainsonine is very scarce and expensive, which is far from meeting the needs of people's research and clinical treatment

Method used

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  • A kind of Ehrigia spinosa for synthesizing swainsonine and its preparation method and application
  • A kind of Ehrigia spinosa for synthesizing swainsonine and its preparation method and application
  • A kind of Ehrigia spinosa for synthesizing swainsonine and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation and screening of fungus Ehrigia spinosa FEL1a-AVB3

[0029] 1) Wash the whole mutant Astragalus membranaceus grown on the Gobi in Minqin County, Gansu Province with deionized water to remove the dust on the surface of the mutant Astragalus membranaceus, use sterilized tweezers to separate the leaves, stems and flowers of the plant, and then separate the leaves, stems, The flowers were wrapped with gauze separately for surface disinfection.

[0030] 2) Use sterilized filter paper to absorb the water on the surface-sterilized plant tissue, then use sterilized scissors to cut leaves, stems, flowers, etc. Each tissue block was inoculated on the surface of water agar medium, and cultured in an incubator at 18°C.

[0031] 3) When the hyphae grow out from around the tissue block, pick the mycelium at the edge of the colony and inoculate it on the surface of the enrichment medium, and place it in an incubator at 18°C ​​for enrichment culture; collect th...

Embodiment 2

[0032] Embodiment 2: the identification of fungus Ehrigia spinosa FEL1a-AVB3

[0033] 1. Morphological features

[0034] The colony of the FEL1a-AVB3 strain was round, white at first, and gradually changed to gray-green, gray-brown, and black with the extension of culture time, with a slight protrusion in the center ( figure 1 ). The mycelium has a transverse septum without a mediastinum, and some old mycelium give birth to conidiophores with few branches. Conidia and conidiophores with septum, without mediastinum, the septum of conidia is thicker, the number of septa ranges from 1 to 4, the spores are rod-shaped, nearly cylindrical, and the size of conidia is 20-80 μm ×6~12μm( figure 2 ).

[0035] 2) Characteristics of culture

[0036]The strain grows very slowly on PDA, PCA, Czapek and other media, and its growth rate is 0.53, 0.60 and 0.40mm / d, respectively. The optimum growth temperature is 18°C, and the growth is inhibited when the temperature is higher than 25°C. ...

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PUM

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Abstract

The invention discloses an Ehrigia spinosa FEL1a-AVB3 for synthesizing swainsonin, which was preserved in China Center for Type Culture Collection on October 8, 2008, and the preservation number is CCTCC NO: M 208142. A method for isolating the strain is also disclosed. The FEL1a-AVB3 strain parasitizes the leaves, stems, flowers, and seeds of the mutant Astragalus membranaceus, and it is symbiotic with the mutant Astragalus membranaceus and does not cause the disease of the mutant Astragalus membranaceus. It was found that FEL1a-AVB3 can produce SW through in vitro culture, indicating that it is involved in the mutant Astragalus membranaceus The biosynthesis of SW is closely related to the toxicity of mutated Astragalus. By inhibiting the growth of FEL1a-AVB3 in mutated Astragalus, the content of SW in mutated Astragalus can be reduced, so as to reduce the poisoning of mutated Astragalus in animals.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a new type of Ehrigia spp. (Embellisaoxytropis), in particular to a type of Ehrysotropis capable of synthesizing the main toxin of madweed, swainsonine (Swainsonine, SW). FEL1a-AVB3 also relates to the preparation and application of the bacteria. Background technique [0002] Locoweed is the general term for the poisonous plants of the genus Oxytropis and Astragalus of the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast grasslands of Northwest, North and Southwest China, with an area of ​​damage reaching 11 million ha 2 , accounting for 2.8% of the country's grassland area. Variation Astragalus is a kind of locoweed Astragalus in my country, mainly distributed in Inner Mongolia, Xinjiang, Gansu, Qinghai, Ningxia and other places; Variation Astraga...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/18A61K31/437A61P35/00C12R1/645
Inventor 王建华余永涛王建娜赵清梅耿果霞
Owner NORTHWEST A & F UNIV
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