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Culture medium for fermenting naringinase, preparation and use method thereof

A technology of culture medium and naringinase, which is applied in the field of culture medium and can solve the problems of low yield

Inactive Publication Date: 2011-11-16
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are domestic reports on the production of naringinase by solid-state fermentation, but the relative yield is relatively low

Method used

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  • Culture medium for fermenting naringinase, preparation and use method thereof
  • Culture medium for fermenting naringinase, preparation and use method thereof
  • Culture medium for fermenting naringinase, preparation and use method thereof

Examples

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Effect test

example 1

[0024] Starch 30g / L, naringin 10g / L, MgSO 4 1g / L, KH 2 PO 4 2g / L, CaCl 2 0.15g / L, (NH 4 ) 2 SO 4 10g / L, ZnSO 4 ·7H 2 O 0.1g / L, yeast extract 6g / L, bean cake powder 10g / L, pH 6.0.

example 2

[0026] Starch 20g / L, naringin 2g / L, MgSO 4 0.2g / L, KH 2 PO 4 0.5g / L, CaCl 2 0.05g / L, (NH 4 ) 2 SO 4 2g / L, ZnSO 4 ·7H 2 O 0.03g / L, yeast extract 0.5g / L, bean cake powder 0.5g / L, pH 6.0.

example 3

[0028] Starch 25g / L, naringin 6g / L, MgSO 4 0.6g / L, KH 2 PO 4 1.3g / L, CaCl 2 0.1g / L, (NH 4 ) 2 SO 4 6g / L, ZnSO 4 ·7H 2 O 0.07g / L, yeast extract 3.3g / L, bean cake powder 6g / L, pH 6.0.

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Abstract

The invention discloses a naringinase fermentation culture medium, a preparation method and a usage method thereof. The naringinase fermentation culture medium has following composition: 20 to 30g / L of starch, 2 to 10g / L of naringin, 0.5 to 6g / L of yeast extract, and 0.5 to 10g / L of bean powder, etc. In the preparation, most parts of starch, yeast extract, bean powder and other raw materials are weighed according to the weight ratio and added into water that occupies 2 / 3 to 3 / 4 of the total volume of a fermented fluid, pH value of the solution is adjusted to be 6.0, and the solution is sterilized and prepared into an initial fermentation culture medium; extra starch, yeast extract, bean powder and all the naringin are added into water that occupies 1 / 4 to 1 / 3 of the volume of a final fermented fluid, and the solution is sterilized and prepared into a material supplementary fluid. The culture medium and the preparation method can be used for preparing high-yield naringinase with the yield of 1100U in each milliliter of the fermented fluid or 55000U in each gram of a solid medium.

Description

technical field [0001] The invention relates to a culture medium, in particular to a culture medium for fermenting naringinase by adopting Aspergillus niger as a strain and using a deep ventilation fermentation technology, and a preparation and use method thereof. Background technique [0002] Naringinase has important industrial application value. It can hydrolyze rhamnoside to produce rhamnose[1], and can hydrolyze prunin into naringin and glucose. It can be used in industry to reduce the bitterness of Rutaceae fruit juice, and can hydrolyze The bonded aromatic substances in grapes can release aromatic substances, which can be used to improve the traditional rhamnose production process. [0003] The production of naringinase by microbial fermentation has long been reported abroad. For example, S.Elinbaum produced naringinase by solid-state fermentation of Aspergillus, and Gallego produced naringinase by submerged culture. There are domestic reports on the production of na...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12R1/685
Inventor 蔡慧农倪辉肖安风黄高凌李利君
Owner JIMEI UNIV
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