Method for injecting leptin in egg white

A technology for egg whites and breeding eggs, applied in the application, poultry industry, animal husbandry and other directions, can solve problems such as the influence of the liver fat metabolism pattern, and achieve the effect of a simple method

Inactive Publication Date: 2009-08-19
NANJING AGRICULTURAL UNIVERSITY
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  • Claims
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Problems solved by technology

Different from mammals, the liver is not only the main place for the synthesis and secretion of leptin in birds, but also the most important fat metabolism organ in birds. However, whether maternal leptin or leptin treatm

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  • Method for injecting leptin in egg white
  • Method for injecting leptin in egg white
  • Method for injecting leptin in egg white

Examples

Experimental program
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Effect test

Example Embodiment

[0047] Example 1

[0048] Three yellow broiler breeding eggs were selected and randomly divided into two groups. The control group was injected with 100μL solvent (PBS) into the egg white, and the test group was injected with 100μL containing 0.5μg mouse recombinant leptin (498-OB-01M, R&D, USA) (Physiol Res 2003) ; 52(2):201-9) PBS. The blood samples and liver tissue samples were taken at 12 and 17 embryonic age and the day of hatching (0 day old) respectively. Radioimmunoassay was used to determine serum and liver leptin concentrations, real-time fluorescent RT-PCR was used to quantify liver LEPR and fat metabolism-related genes, and Western blot was used to determine liver LEPR protein content. The results showed that the chick weight in the leptin treatment group was significantly reduced (P=0.000), while the liver body index was significantly increased (P=0.018), especially the difference between females was extremely significant (both chick weight and liver body index were P...

Example Embodiment

[0065] Example 2

[0066] Three-yellow broiler breeding eggs with similar egg weights were selected in this experiment and randomly divided into three groups: the control group (control, Con) was injected with 100μL solvent (PBS) into the egg white, and the leptin treatment group was injected with 0.5μg / 100μL PBS (low dose group) , LL) and 5.0μg / 100μL PBS (high-dose group, HL) mouse recombinant leptin, and liver and serum samples were taken when the chicks were raised to 21 days of age. Radioimmunoassay was used to determine the concentration of leptin and thyroid hormones in the liver and serum, Westernblot was used to determine the content of LEPR protein in the liver of chickens, and real-time fluorescent RT-PCR was used to quantify the expression of genes related to liver LEPR and fat metabolism. The results showed that intra-egg injection of leptin affected the early growth of broilers after hatching, and showed sex-specific and dose-dependent (see Table 2-1, 2-2, 2-3). Regar...

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Abstract

The invention discloses a method for injecting leptin into egg white, and belongs to the technical field of animal growth regulation. The method comprises: before incubation, disinfecting the small end of a hatching egg; drilling a hole lightly on the disinfected part by a toad marrow puncture needle; injecting leptin injection reagent into the egg white through the hole by a micro injector; and sealing the hole by paraffin. After the leptin with certain concentration is injected into the egg white, the leptin is observed to inhibit the development of embryo of table poultry and reduce body weight of newly hatched offspring, but promote early growing development of the table poultry after hatch, and the leptin can be taken as semiochemicals to mediate the routinization effect on the early growing development of filial generations of the table poultry.

Description

1. Technical field [0001] The invention relates to a method for injecting leptin into egg white. Specifically, the invention relates to a method of using leptin as a signal substance to mediate the programmed effect on the early growth and development of broiler offspring, and belongs to the technical field of animal growth regulation. 2. Background technology [0002] In the 21st century, obesity has become an important public health problem and has attracted more and more attention from the world. With the development of economy and the improvement of material life in our country, more and more children have quietly joined the ranks of obese people. Obese children have accounted for 10% of the total number of children, and are increasing at an annual rate of 8%. Today, the requirements for animal food quality are increasing day by day, because the lipid deposition of meat livestock and poultry is directly related to the quality of meat products, the problem of animal obesi...

Claims

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Application Information

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IPC IPC(8): A01K67/02A01K45/00
Inventor 赵茹茜胡艳
Owner NANJING AGRICULTURAL UNIVERSITY
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