Method for extracting gibberellin by salting-out method

Abstract

The invention provides a method for extracting gibberellin from ferment filtrate by a salting-out method, comprising the following steps of adding adequate sodium chloride or sodium sulfate in gibberellin-contained ferment filtrate, separating the gibberellin out of the filtrate, and obtaining the crude extract containing the gibberellin by solid-liquid separation; wherein the yield of the crude extract is more than 98%.

Description

Technical field: [0001] The invention describes an extraction method of an agricultural plant growth regulator, specifically extracting gibberellin from a fermentation filtrate by a salting-out method. Background technique [0002] Gibberellin is a well known plant growth regulator. The gibberellins used in production are produced by liquid fermentation using Gibberella. The extraction process is: after the fermentation is finished, the fermentation liquid is filtered through a plate frame to obtain a gibberellin-containing fermentation filtrate and a mycelium filter cake; there are two methods for further extracting gibberellin from the fermentation filtrate: one is to use ethyl acetate The gibberellin is extracted directly with the ester; the second is to first concentrate the fermentation filtrate to 1 / 5 volume under reduced pressure, and then use ethyl acetate to extract the gibberellin in the concentrated solution. Use the first method to extract gibberellin, the extr...

AI Technical Summary

Problems solved by technology

Use the first method to extract gibberellin, the extraction rate is high but the consumption of organic solvent is large; the disadvantage of using the second method to extract gibberellin from the fermentation filtrate is that in the process of heating and decompression concentration, the pH value is slightly lower. Offset or too high temperature or too long concentration time will cause the degradation of gibberellin and reduce the yield