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Liquid medium pollen, preparing method and application thereof

A liquid medium and pollen technology, applied in the direction of botanical equipment and methods, applications, chemicals for biological control, etc., can solve problems that have not been reported yet, so as to improve pollen vigor, increase fruit setting rate, and reduce seed production cost effect

Inactive Publication Date: 2009-10-14
YUNNAN ACAD OF TOBACCO AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the relevant research and application of polyamine liquid medium pollen on crops has not yet been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The selected demonstration field is located in the seed production base of China Tobacco Seed Co., Ltd. in Yuxi, Xishuangbanna, Yunnan. Tobacco seedlings are raised by floating seedlings, which are then transplanted to the field after they grow into seedlings. Field planting density: row spacing 1.2m, plant spacing 0.5m.

[0025] Field implementation of 10 mu of land was carried out in the seed production base. Among them, 2 mu are used to plant the male parent of Yunyan 87, and the remaining 8 mu are used to plant the female parent of Yunyan 87. When the flower of the male parent of Yunyan 87 reached the flower-picking standard, the pollen collection began. The anthers of the male parent of Yunyan 87 were collected and dried to loosen the powder. The pollen was sieved with a 50-mesh sieve, and the collected pollen was sealed and stored at 4°C for later use. According to the ratio of 50mg / ml, the pure pollen was weighed and added to the putrescine solution with a conc...

Embodiment 2

[0027] Example 1 was repeated with the following difference: the 0.2 mM putrescine solution was replaced with a 0.2 mM spermidine solution.

Embodiment 3

[0029] Repeat Example 1 with the following difference: the 0.2 mM putrescine solution was replaced with a 0.2 mM spermine solution.

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Abstract

The invention discloses liquid medium pollen, a preparing method and applications thereof. The invention aims at improving the activity and the quality of pollen, reducing the use level of pure pollen and reducing the cost of seed production. Tobacco pure pollen and 0.2 to 0.5 mM polyamine solution are mixed, the mixture ratio of the pollen to the solution is 50 to 80 mg / ml, and the liquid medium pollen is obtained after uniform fixing. The polyamine solution is selected form one of putrescine, spermidine or spermine. Simultaneously the invention provides the application of the liquid medium pollen in tobacco artificial pollination.

Description

technical field [0001] The invention belongs to the technical field of artificial pollination, and more specifically, the invention relates to a liquid medium pollen for tobacco hybrid seed production. At the same time, the invention also relates to the preparation method and application of the liquid medium pollen. Background technique [0002] In order to protect the intellectual property rights of breeders and achieve the purpose of controlling the planting area of ​​tobacco leaves, my country currently promotes the use of male sterile varieties of tobacco in a large area of ​​tobacco leaf production. The planting area is expanding year by year, and the amount of seeds used is also increasing year by year. According to incomplete statistics, in 2007, the area of ​​promoting the cultivation of tobacco sterile lines in the national tobacco production has exceeded 530,000 hectares (see Zhejiang Agricultural Science, 2009, 1: 22-25 for details). The popularization and planti...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01N33/04A01P21/00
Inventor 马文广郑昀晔李永平肖江海邓盛斌索文龙阚继亮薛大章宋碧清
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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