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Cinobufagin injection effective component and use thereof in preparation of antineoplastic drug

A technology for effective components and injections, which is applied to the effective components of cinobufacin injection and the new application field in the preparation of anti-tumor drugs

Inactive Publication Date: 2009-10-14
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about the antitumor effect of cinobufacin on pancreatic cancer

Method used

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  • Cinobufagin injection effective component and use thereof in preparation of antineoplastic drug
  • Cinobufagin injection effective component and use thereof in preparation of antineoplastic drug
  • Cinobufagin injection effective component and use thereof in preparation of antineoplastic drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Extraction, separation and identification of effective components of cinobufacin injection

[0036] 1.1 Instruments and medicines

[0037]HP-20 macroporous resin (Mitsubishi Chemical); chromatography column (diameter 5cm×10cm); vacuum decompression concentration tank (Jiangyin Dinghong Machinery Equipment Co., Ltd.); silica gel thin-layer chromatography plate (Yantai Huiyou Silica Gel Development Co., Ltd. Company); thin-layer chromatography development cylinder; UV-I type ultraviolet analyzer (Beijing Likang Dasheng Technology Co., Ltd.); high-performance liquid chromatography Agilent 1100 system, including quaternary pump, DAD detector, manual injector. Cinobufacin injection 20-fold concentrated solution (Anhui Jinchan Biochemical Co., Ltd.), the concentration of which is equivalent to 10g of the original drug per milliliter of the drug solution. Properties: This product is a dark brown liquid with a slightly fishy smell and a bitter taste; cinobufacin (Anh...

Embodiment 2

[0056] Antitumor effects of different components of cinobufacin injection on human pancreatic cancer SW1990 in vivo and in vitro

[0057] 1.1 Materials

[0058] Human pancreatic cancer cell line SW1990 (purchased from the Cell Bank of Experimental Animal Center of Sun Yat-sen University). CCK-8 kit was purchased from Tongren Institute of Chemistry, DMSO was purchased from Sigma Company, RPMI 1640 culture medium and fetal bovine serum were purchased from Gibco Company. 104 BALB / C-nu / nu nude mice, SPF grade, female, weighing 18-22 g, were purchased from Slack Experimental Animal Co., Ltd., Shanghai Academy of Life Sciences, Chinese Academy of Sciences. Cinobufacin injection and 20 times concentrated solution (Anhui Jinchan Co., Ltd., batch number 042355, 050104). The different components separated from cinobufacin injection were prepared according to the method in Example 1.

[0059] 1.2 Separation of different components of cinobufaginin

[0060] Put 400ml of the 20-fold co...

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Abstract

The invention relates to the technical field of Chinese traditional medicine preparation, in particular to cinobufagin injection effective component and new use of the effective component in preparation of antineoplastic drug. The invention uses macroporous absorbent resin to separate effective components from cinobufagin injection; water and 50% ethanol are then respectively to use for eluting components of various polarities separated by HP-20 macroporous absorbent resin; the components are then analyzed and distinguished by thin layer chromatography and high performance liquid chromatography to prepare non-polarity components of cinobufagin injection with lipophilic phrynin lactone substances and polarity components of water-soluble alkaloid substances. In vivo and in vitro anti-tumor tests of the cinobufagin injection effective component has proven that the cinobufagin injection effective component can inhibit SW1990 auxesis; the cinobufagin injection effective component has obvious inhibiting effect to tumor growth of nude mice loaded with human pancreatic cancer which can be used for further preparation of anti-human pancreatic cancer drug, in particular for preparation of anti-human pancreatic cancer drug which comprises non-polarity components of cinobufagin injection.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to an effective component of cinobufacini injection and its new application in the preparation of antitumor drugs. Background technique [0002] Cinobufacin injection (Huchansu injection) is a water-soluble preparation made from Bufo gargarizans cantor or B. melanosticutus schneider. Primary liver cancer, lung cancer, esophageal cancer and gastric cancer have better curative effect (Zuo Xiaodong, Cui Yongan, Qin Shukui etc. Journal of Clinical Oncology, 2003, 8 (3): 232-235). Prior art discloses, Cinobufacin has obvious inhibitory effect on the growth of human gastric adenocarcinoma MGC-80-3 cells and leukemia U937 cells, and its IC50 is about 0.16ug / ml and 1.36ug / ml respectively; The growth of -7 cells and human leukemia NB4 cells has obvious inhibitory effect (Zuo Xiaodong, Cui Yongan, Wang Jinhong, etc. Journal of Clinical Oncology, 2003, 8(1): 33-37; Wang Shao...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/56A61P35/00A61K35/65
Inventor 朱晓燕刘鲁明孟志强陈震俞培忠葛勇前
Owner FUDAN UNIV SHANGHAI CANCER CENT
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