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Process combination for extracting polysaccharide in hericium erinaceus mycelium cells and determination method thereof

A technology of Hericium erinaceus and intracellular polysaccharides, applied in the field of biopharmaceutical production, can solve problems such as product separation difficulties and increase production costs, and achieve the effects of improving separation efficiency, increasing yield, and protecting the active ingredients of polysaccharides

Inactive Publication Date: 2009-10-21
ZHEJIANG UNIV
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Problems solved by technology

However, since the enzyme extraction will increase the production cost and also bring difficulties to the separation of the product, the amount of addition should be strictly controlled

Method used

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  • Process combination for extracting polysaccharide in hericium erinaceus mycelium cells and determination method thereof

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Embodiment Construction

[0021] Zhejiang Qingyuan County Fangge Medicinal Mushroom Co., Ltd. produces Hericium erinaceus mycelium dry powder polysaccharide extraction process combination. Its composition is: the ratio of material to liquid is 1:30 by mass ratio, the amount of compound enzyme added is 2%, ultrasonic The power is 200W, the enzymolysis time is 50min, the enzymolysis temperature is 55°C, and the enzymolysis pH is 5.0.

[0022] A method for determining the polysaccharide extraction process combination in the mycelium of Hericium erinaceus is as follows:

[0023] 1) The dry powder of Hericium erinaceus mycelium produced by Fangge Medicinal Mushroom Company in Qingyuan County, Zhejiang Province was selected as the raw material for extraction.

[0024] 2) Determine the ratio of material to liquid

[0025] Polysaccharides were extracted by hot water extraction. Select 100g of mycelium dry powder from step 1), take the water addition multiple as the analysis factor, set 10, 20, 30, 40, 50, 60...

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Abstract

The invention relates to a process combination for extracting polysaccharide in hericium erinaceus mycelium cells. The solid-liquid ratio according to weight is 1: 10-1: 60, the adding amount of complex enzyme accounts for 0.5 percent to 5.0 percent of the weight of the raw materials, the range of ultrasonic power is 100w to 500w, the range of enzymolysis time is 40min to 100min, and the range of enzymolysis temperature is 30 DEG C to 60 DEG C, and the range of enzymolysis pH value is 4.0 to 8.0. The determination method of the process combination for extracting polysaccharide in hericium erinaceus mycelium cells is as follows: high-quality mycelium is selected to be deeply fermented, thus obtaining mycelium dry powder; the solid-liquid ratio is determined; the adding amount of complex enzyme is determined; and the optimal extracting process combination for extracting mycelium dry powder polysaccharide in an ultrasonic-complex enzyme method is determined.

Description

technical field [0001] The invention relates to biopharmaceutical production technology, in particular to a process combination for extracting intracellular polysaccharides from Hericium erinaceus mycelia and a determination method thereof. Background technique [0002] Hericium erinaceus is a well-known medicinal and edible fungus. It uses fruiting bodies as medicine. It is flat in nature and sweet in taste. It has the functions of helping digestion and benefiting the five internal organs. Good medicine for gastrointestinal diseases. It is a "high-protein, low-fat" food and a good health fungus. [0003] Hericium erinaceus polysaccharides are widely used in medicine, food and other fields, and the market demand is increasing day by day. However, at present, the production of Hericium erinaceus polysaccharides is almost all extracted from fruiting bodies. Wild resources of Hericium erinaceus are scarce, and the artificial cultivation period is long and occupies an area of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 李卫旗
Owner ZHEJIANG UNIV
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