Process combination for extracting polysaccharide in hericium erinaceus mycelium cells and determination method thereof

A technology of Hericium erinaceus and intracellular polysaccharides, applied in the field of biopharmaceutical production, can solve problems such as product separation difficulties and increase production costs, and achieve the effects of improving separation efficiency, increasing yield, and protecting the active ingredients of polysaccharides

Inactive Publication Date: 2009-10-21
ZHEJIANG UNIV
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the enzyme extraction will increase the production cost and also bring difficulties to the separation of the product, the amount of addition should be strictly controlled

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process combination for extracting polysaccharide in hericium erinaceus mycelium cells and determination method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] Zhejiang Qingyuan County Fangge Medicinal Mushroom Co., Ltd. produces Hericium erinaceus mycelium dry powder polysaccharide extraction process combination. Its composition is: the ratio of material to liquid is 1:30 by mass ratio, the amount of compound enzyme added is 2%, ultrasonic The power is 200W, the enzymolysis time is 50min, the enzymolysis temperature is 55°C, and the enzymolysis pH is 5.0.

[0022] A method for determining the polysaccharide extraction process combination in the mycelium of Hericium erinaceus is as follows:

[0023] 1) The dry powder of Hericium erinaceus mycelium produced by Fangge Medicinal Mushroom Company in Qingyuan County, Zhejiang Province was selected as the raw material for extraction.

[0024] 2) Determine the ratio of material to liquid

[0025] Polysaccharides were extracted by hot water extraction. Select 100g of mycelium dry powder from step 1), take the water addition multiple as the analysis factor, set 10, 20, 30, 40, 50, 60...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a process combination for extracting polysaccharide in hericium erinaceus mycelium cells. The solid-liquid ratio according to weight is 1: 10-1: 60, the adding amount of complex enzyme accounts for 0.5 percent to 5.0 percent of the weight of the raw materials, the range of ultrasonic power is 100w to 500w, the range of enzymolysis time is 40min to 100min, and the range of enzymolysis temperature is 30 DEG C to 60 DEG C, and the range of enzymolysis pH value is 4.0 to 8.0. The determination method of the process combination for extracting polysaccharide in hericium erinaceus mycelium cells is as follows: high-quality mycelium is selected to be deeply fermented, thus obtaining mycelium dry powder; the solid-liquid ratio is determined; the adding amount of complex enzyme is determined; and the optimal extracting process combination for extracting mycelium dry powder polysaccharide in an ultrasonic-complex enzyme method is determined.

Description

technical field [0001] The invention relates to biopharmaceutical production technology, in particular to a process combination for extracting intracellular polysaccharides from Hericium erinaceus mycelia and a determination method thereof. Background technique [0002] Hericium erinaceus is a well-known medicinal and edible fungus. It uses fruiting bodies as medicine. It is flat in nature and sweet in taste. It has the functions of helping digestion and benefiting the five internal organs. Good medicine for gastrointestinal diseases. It is a "high-protein, low-fat" food and a good health fungus. [0003] Hericium erinaceus polysaccharides are widely used in medicine, food and other fields, and the market demand is increasing day by day. However, at present, the production of Hericium erinaceus polysaccharides is almost all extracted from fruiting bodies. Wild resources of Hericium erinaceus are scarce, and the artificial cultivation period is long and occupies an area of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 李卫旗
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap