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Technology for preparing lactose enzyme

A technology of lactase and preparation, which is applied in the field of microbial technology to prepare industrial enzyme preparations, can solve the problems of dependence on imports, and achieve the effects of low production cost, short cultivation time, and extended product stability period

Inactive Publication Date: 2009-11-04
JIAMI SHANGHAI BIOLOGICAL ENG
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Problems solved by technology

[0006] Since the 1970s, foreign countries have been engaged in the research and development and production of lactase, while the research and development and production of lactase in my country is still in a blank stage, and the country is currently in a situation of complete dependence on imports.

Method used

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  • Technology for preparing lactose enzyme

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Embodiment Construction

[0040] The present invention will be further described below in conjunction with specific examples, but the present invention is not limited.

[0041] 1) Implement the preparation of lactase

[0042] 1-1 The Aspergillusoryzae ATCC 6061 strain purchased from Japan Society For Culture Collections was soaked at a chemical NTG concentration of 100-1000 μg / ml for 20-40 minutes, repeated 4-8 times. Lactase preparation was performed after incubation in 500 ml shake flasks.

[0043] 1-2 Inoculate the strains into the sterilized seed medium for fermentation, the seed medium is (weight%) glucose 2%, peptone 1%, yeast essence 0.5%, ammonium phosphate 0.01%, add water to make the required volume , 0.1MP sterilization for 20 minutes, the seed culture temperature is 32-42°C, and the culture time is 16-40 hours. The seed culture filling volume is 200ml / 500ml triangular flask, and the box type reciprocating shaker is 200rpm. After the seed culture is fermented, the bacterial classification ...

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Abstract

The invention provides a method for preparing lactose enzyme which is a novel high-efficient preparation. In the method, bacteria are processed by NTG with a certain concentration to produce high yield bacteria, solid fermentation is carried out under the condition which is suitable for the growth of microorganisms of the high yield bacteria, and industrial enzyme with high purity quotient and ideal yield coefficient is obtained by extracting and separating under a totally enclosed condition. The method has the advantages of soft preparing condition and little generation of three wastes.

Description

technical field [0001] The invention relates to the preparation of industrial enzyme preparations by utilizing microbial technology, more specifically refers to the preparation of lactase by using aspergillus oryzae through microbial technology. Background technique [0002] The enzyme preparation industry is a knowledge-intensive high-tech industry. The application fields of enzyme preparations are extremely wide. Enzymes have the characteristics of high efficiency and specific reaction conditions. For example, hydrolysis, redox, cleavage, chemical group transfer, etc. make the object undergoes a chemical reaction according to the predetermined assumption to produce the desired target product. Enzymes are proteins that are completely biodegradable by themselves. As a specific and efficient biocatalyst, enzymes can replace traditional chemical and physical processes, such as replacing chemical reagents, reducing industrial water consumption, reducing energy consumption, red...

Claims

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Application Information

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IPC IPC(8): C12N9/38C12R1/69
Inventor 林澄敏林再庆
Owner JIAMI SHANGHAI BIOLOGICAL ENG
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