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Polychromatic, diversely-sized particles for angiography

A particle and blood vessel technology, applied in the field of detection of blood vessel leakage and blood flow, can solve the problems of leaking retina, subspace, fluorescein leakage, etc.

Inactive Publication Date: 2009-12-30
THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in diseased eyes, the blood-retinal barrier is damaged, leading to leakage of fluorescein into the vitreous in the case of diabetic retinopathy, or into the subretinal space in the case of age-related macular degeneration

Method used

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  • Polychromatic, diversely-sized particles for angiography
  • Polychromatic, diversely-sized particles for angiography
  • Polychromatic, diversely-sized particles for angiography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1 Materials and methods

[0075] Materials: Poly(D,L-lactide-co-glycolide) (PLGA), Mw = 10,000 Da (intrinsic viscosity: 0.17 dL / g), purchased from Birmingham Polymers (Durect Corporation, Pelham, AL). Indocyanine green, sodium fluorescein and poly(vinyl alcohol) (PVA) were obtained from Sigma-Aldrich (St Louis, MO). Dichloromethane was obtained from Acros (Morris Plains, NJ). All other chemicals were of analytical grade and obtained from local sources.

[0076]Nanoparticle formulation: PLGA nanoparticles were prepared using a slightly modified nanoprecipitation technique (Chorny et al., 2002, Journal of Controlled Release). Briefly, polymer (70 mg) and indocyanine green or fluorescein (30 mg) were dissolved in a mixture of 15.5 mL acetone, 4 mL ethanol and 0.5 mL dichloromethane and poured into the filtered 1% In the PVA aqueous phase, distilled water (40 ml) was added under moderate stirring. After evaporating the organic phase overnight at 37°C using a ...

Embodiment 2

[0082] Example 2: Detection of blood-retinal barrier dysfunction

[0083] This example shows the detection of blood-retinal barrier damage using a composition of differently sized, differently labeled particles.

[0084] Filtration studies illustrate different particle sizes

[0085] Such as Figure 5 As shown in , particle formulations of different particle sizes were produced with different chromophores. The separation and detection of particles of different sizes is effected by the filtration of membranes with different pore sizes. Therefore, a composition of two particle groups was prepared, as Figure 5 shown in (lower row, far right). The composition is shown in Figure 5 (lower row, middle). Compositions comprising smaller, fluorescein-labeled PLGA particles are shown in separate form at Figure 5 (lower row, left), and larger indocyanine green PLGA particles are shown in Figure 5 (bottom, right). Thus, the composition comprises a mixture of fluorescein PLGA a...

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Abstract

The invention involves polychromatic particles of various sizes for assessing blood flow, blood barrier leakage and blood vessel leakage.

Description

[0001] This application claims priority from US Provisional Application Serial No. 60 / 806,711, filed July 6, 2006, the contents of which are incorporated herein by reference. field of invention [0002] The present invention relates to compositions for use in angiography and methods for detecting vascular leakage and blood flow. In some embodiments, the compositions and methods of the invention can be used to assess the integrity of the blood-retinal barrier and / or quantify the extent of damage within the blood-retinal barrier. Thus, the compositions of the present invention can be used as contrast agents in two-dimensional, three-dimensional imaging and similar techniques. Background of the invention [0003] Retinal angiography is a technique used in ophthalmology to study the physiological and pathological conditions of the eye. For example, angiography has proven useful in both research and clinical settings for the assessment of retinal diseases, such as diabetic retin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K51/12
CPCA61K49/0043A61K49/0093B82Y5/00A61K51/1244A61K49/0002A61K49/0034
Inventor S·R·塔里G·R·巴里莱U·B·科姆佩拉
Owner THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK