Pollen suspension suitable for liquid pollination of fruit trees and preparation method thereof

A pollen suspension and liquid pollination technology, applied in the directions of plant cells, cultivation, application, etc., can solve the problem of pollen loss of viability, and achieve the effects of saving labor, promoting fruit setting, and improving pollination quality.

Inactive Publication Date: 2010-01-06
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a pollen suitable for liquid pollination of fruit trees in view of the fact that the liquid pollination solution (mainly the mixed solution of sucrose, boron and other components

Method used

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  • Pollen suspension suitable for liquid pollination of fruit trees and preparation method thereof
  • Pollen suspension suitable for liquid pollination of fruit trees and preparation method thereof
  • Pollen suspension suitable for liquid pollination of fruit trees and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Screening of pollen suspended components

[0024] Aiming at the problems of poor self-flowering, poor pollination quality and low fruit setting rate of the apricots used in the experiment, we want to improve their pollination status through liquid pollination. First adopt conventional method to configure pollen suspension namely: 10% sucrose+0.3% boric acid+0.3% pollen sprays apricot tree, but fruit set rate is very low, also lower than the tree body of contrast (not spraying). It shows that this method has no effect on promoting apricot fruit setting. After careful observation, it was found that the pollen precipitated soon after the prepared liquid was put in. Therefore, we started the screening of ingredients that can make the pollen well suspended. Six kinds of solutions were prepared in this experiment (the following concentrations are weight percent concentrations): (1) 5% sucrose solution; (2) 10% sucrose solution; (3) 0.1% agar+5% sucrose mixed solu...

Embodiment 2

[0025] Example 2: Component Screening for Maintaining Pollen Viability in Liquid

[0026] After confirming the effect of the mixed solution of 0.04% xanthan gum + 10% sucrose on pollen suspension, how to keep the pollen in the liquid for a long time is another question we discuss. To this we have designed following experiments (the following concentrations are weight percent concentrations): (1) 0.04% xanthan gum+10% sucrose mixed solution; (2) 0.04% xanthan gum+10% sucrose+0.3% boron mixed Solution; (3) 0.04% xanthan gum + 10% sucrose + 0.01% pectin methylesterase mixed solution, pollen concentration is 0.3%. After the pollen solution was mixed with the above solution, place it still. At 0, 15, 30, 60, and 90 min, 200 μL of the solution was taken from the bottom for the pollen germination experiment (medium formula: 1% agar + 10% sucrose, 2 ~3h), calculate the pollen germination rate under the microscope, and repeat 3 times. The result is as figure 2 Shown: It can be seen...

Embodiment 3

[0028] Embodiment 3: Practical application effect

[0029] According to the above formula, we conducted a pollination experiment on the golden sun apricot at the full flowering stage in the hot spring nursery in Haidian District, Beijing. Four treatments were set up in the experiment (the following concentrations are weight percentages), that is, treatment 1 was 10% sucrose+0.3% boron mixed solution; treatment 2 was 0.1% agar+10% sucrose+0.3% boron mixed solution; treatment 3 was Mixed solution of 0.04% xanthan gum + 10% sucrose + 0.01% pectin methylesterase; treatment 4 was artificial spot infusion, and the pollen concentration of liquid pollination was 0.3%. The result is as image 3shown. It can be seen from the figure that the fruit setting rate of artificial spot-infected trees is the highest, and the pollen suspension formula of 0.04% xanthan gum + 10% sucrose + 0.01% pectin methylesterase in liquid pollination has the highest fruit-setting rate, second only to artific...

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Abstract

The invention discloses pollen suspension suitable for the liquid pollination of fruit trees and a preparation method thereof, belonging to the technical field of pomiculture. The required pollen suspension is obtained by two steps, namely sieving pollen suspended components and sieving components keeping the viability of pollen in liquid. The invention can well suspend and evenly distribute the pollen to be well suspended, and the pollen can keep higher viability in a liquid solution; the invention can be widely applied to the liquid pollination of fruit trees and can realize the manual liquid pollination operation with large area, thereby overcoming the difficulty of sterilitas of the self flowers or low fruition rate of the self flowers of tree species in production; and the invention enhances the pollination quality, promotes fructification, saves labour and enhances the efficiency.

Description

technical field [0001] The invention relates to a pollen suspension suitable for liquid pollination of fruit trees and a preparation method thereof, belonging to the technical field of fruit tree cultivation. Background technique [0002] For fruit trees with low self-fertilization or self-fertilization rate, the cultivation method of configuring pollination trees is often used in production at present. This requires that the configured pollination tree species have the same flowering period as the main species, and have a high pollination affinity with the main species. Even so, since pollination trees are planted in fixed areas, they are immobile, and in production, due to differences in distance or orientation, problems such as uneven pollination, no pollination, and poor pollination quality often occur between trees. Poor pollination will lead to low fruit setting rate, irregular fruit shape, and poor fruit quality. [0003] Artificial pollination is an important techn...

Claims

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Application Information

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IPC IPC(8): A01G17/00C12N5/04
Inventor 胡建芳李天红廖康王涛
Owner CHINA AGRI UNIV
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