Kit for quantitatively detecting ABL mRNA level

Abstract

The invention discloses a kit for quantitatively detecting ABL mRNA level. The kit comprises a standard product for manufacturing a standard curve and an internal reference gene real-time quantitative PCR system. The kit can accurately, rapidly and quantitatively detect the ABL mRNA level. In various leukaemia fusion gene detections, ABL is taken as the internal reference gene, the kit is used for performing quantitation of the internal reference gene to realize accurate, rapid and quantitative detection of various leukaemia fusion gene, further accurately and rapidly performs molecular diagnosis of leukaemia and monitoring of minimal residual diseases during treatment, and provides important molecular basis for confirmed diagnosis of clinic diseases, determination of treatment proposals, curative effect evaluation and prognosis.

Description

technical field [0001] The invention relates to a kit for quantitatively detecting the level of ABL mRNA. Background technique [0002] The real-time quantitative PCR technology (RQ-PCR) invented in recent years has realized the leap from qualitative to real quantitative PCR. Compared with ordinary PCR, RQ-PCR has the following advantages in addition to being able to quantify: (1) By adding probes to the PCR reaction system or by making a melting curve, the specificity and sensitivity are enhanced; (2) the amplification Closed tube operation during the process collects data in real time, reducing chances of contamination and false positive results; (3) no need for electrophoresis, shortening operating time; (4) evaluating sample quality by quantifying internal reference genes, reducing false negative results. The key point of the RQ-PCR technology based on TaqMan probes is that in addition to the upstream and downstream primers in the PCR reaction system, TaqMan probes are ...

AI Technical Summary

Problems solved by technology

[0003] To calculate the amount of starting template, a standard curve must be used. The standard curve is made by RQ-PCR using serially diluted standards of known starting amount. The standard can be cDNA of known quality or known copy number. The former is relatively simple to make, but the result of the unknown sample obtained is not intuitive and difficult to understand; while the preparation of the plasmid standard is cumbersome, but it can be used to calculate the copy number of the unknown sample, and the result is intuitive, which is currently the most widely used plasmid. Wide range of standard types

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