Gastrodiaelata Blume plant extract for preventing and treating vascular dementia and preparation method thereof

A plant extract and a technology for vascular dementia are applied in the field of preparing medicines for preventing and treating vascular dementia, and can solve problems such as no report on the plant extract of Gastrodia elata, no discovery, etc.

Active Publication Date: 2010-01-20
北京协和制药二厂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Due to the complex chemical composition in Gastrodia elata and the large difference in the polarity of the paresin compounds, it is difficult to enrich high-content (more than 50%) paresin derivatives with a single chromatographic separation technology suitable for industrial production. There is no bibliographical report that has obtained the Gastrodia elata plant extract of high-content (greater than 50%) parisin derivatives, and there is no report that utilizes chromatographic separation technology to realize the method for preparing the gastrodia elata plant extract rich in parisin and its derivatives , and it has never been found that the Gastrodia elata plant extract containing Perisin derivatives or the monomeric compounds of Peresin derivatives (especially Peresin A, B, C) or mixtures have the effects of increasing intelligence and preventing and treating vascular dementia

Method used

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  • Gastrodiaelata Blume plant extract for preventing and treating vascular dementia and preparation method thereof
  • Gastrodiaelata Blume plant extract for preventing and treating vascular dementia and preparation method thereof
  • Gastrodiaelata Blume plant extract for preventing and treating vascular dementia and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Extraction: 1 kg of Gastrodia elata, crushed and soaked in 50% ethanol for 3 times, 24 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.

[0048] 2. Refining: the above-mentioned filtrate is eluted by gradient elution with water and 10%, 20%, 30%, 40%, 50%, 75% ethanol through the treated HPD-100 macroporous adsorption resin, detected by HPLC, and collected rich in Fractions of palicine compounds were combined, concentrated under reduced pressure, and spray-dried to obtain 4.48 g of the plant extract. As determined by high performance liquid chromatography, the content of parisin compounds is 51.8%.

Embodiment 2

[0050] 1. Extraction: 1 kg of Gastrodia elata medicinal material, pulverized, added with 50% ethanol, soaked at 50°C for 3 times, 6 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.

[0051] 2. Refining: the above-mentioned filtrate is eluted by gradient elution with water and 10%, 20%, 30%, 40%, 50%, and 75% ethanol through the treated HP-20 type macroporous adsorption resin, detected by HPLC, and collected rich in Fractions of palicine compounds were combined, concentrated under reduced pressure, and spray-dried to obtain 4.42 g of the plant extract. As determined by high-performance liquid chromatography, the content of parisin compounds is 52.6%.

Embodiment 3

[0053] 1. Extraction: 1 kg of Gastrodia elata, a traditional Chinese medicine, was pulverized and soaked in 50% ethanol overnight, then heated and refluxed for 3 times, 3.0 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.

[0054] 2. Refining: the above-mentioned filtrate is washed with water, 10%, 20%, 30%, 40%, 50% and 75% concentration of methanol successively through the treated HP-20 macroporous adsorption resin, detected by HPLC, and collected rich Fractions containing parisin compounds were combined, concentrated under reduced pressure, and spray-dried to obtain 4.68 g of the plant extract. As determined by high performance liquid chromatography, the content of parisin compounds is 54.4%.

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Abstract

The invention relates to application of a Gastrodiaelata Blume plant extract containing parishin derivants on preparing a medicament for preventing and treating vascular dementia and other diseases. The invention also relates to a method for preparing the plant extract extracted and refined from Gastrodiaelata Blume plant, wherein active components are the parishin derivants, in particular to parishin A, B and C. The method adopts alcohol extraction; and an extract is refined and purified by a chromatogram method to obtain the plant extract with the content of the parishin derivants of 35 percent, in particular over 50 percent. A medicament preparation optimally selects injections, troches, capsules, soft capsules, oral liquid or dropping pills and the like.

Description

technical field [0001] The invention belongs to the technical field of natural medicines. It specifically relates to the use of Gastrodia elata Blume plant extract containing palicin A, B, C and / or derivatives thereof in the preparation of medicines for preventing and treating vascular dementia. The plant extract extracted and refined from the traditional Chinese medicine Gastrodia elata and its extraction method. Background technique [0002] Modern lifestyles lead to an increasing number of patients with vascular dementia. Modern medical research believes that vascular dementia (VD) is a multifactorial disease, which is related to factors that cause atherosclerosis such as hypertension, diabetes, and hyperlipidemia. Most scholars believe that the occurrence of vascular dementia is often closely related to cortical lesions, especially left cortical ischemia and ischemic changes in the thalamus and hippocampus. In addition, bilateral, multiple cerebral infarctions, ischemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8988A61K31/7032A61P25/28
Inventor 王珂石建功刘晔赵立敏张猛陈明霞
Owner 北京协和制药二厂有限公司
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