Gastrodiaelata Blume plant extract for preventing and treating vascular dementia and preparation method thereof
A plant extract and a technology for vascular dementia are applied in the field of preparing medicines for preventing and treating vascular dementia, and can solve problems such as no report on the plant extract of Gastrodia elata, no discovery, etc.
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Embodiment 1
[0047] 1. Extraction: 1 kg of Gastrodia elata, crushed and soaked in 50% ethanol for 3 times, 24 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.
[0048] 2. Refining: the above-mentioned filtrate is eluted by gradient elution with water and 10%, 20%, 30%, 40%, 50%, 75% ethanol through the treated HPD-100 macroporous adsorption resin, detected by HPLC, and collected rich in Fractions of palicine compounds were combined, concentrated under reduced pressure, and spray-dried to obtain 4.48 g of the plant extract. As determined by high performance liquid chromatography, the content of parisin compounds is 51.8%.
Embodiment 2
[0050] 1. Extraction: 1 kg of Gastrodia elata medicinal material, pulverized, added with 50% ethanol, soaked at 50°C for 3 times, 6 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.
[0051] 2. Refining: the above-mentioned filtrate is eluted by gradient elution with water and 10%, 20%, 30%, 40%, 50%, and 75% ethanol through the treated HP-20 type macroporous adsorption resin, detected by HPLC, and collected rich in Fractions of palicine compounds were combined, concentrated under reduced pressure, and spray-dried to obtain 4.42 g of the plant extract. As determined by high-performance liquid chromatography, the content of parisin compounds is 52.6%.
Embodiment 3
[0053] 1. Extraction: 1 kg of Gastrodia elata, a traditional Chinese medicine, was pulverized and soaked in 50% ethanol overnight, then heated and refluxed for 3 times, 3.0 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat and dissolve, cool to room temperature, let stand for 24 hours, centrifuge and filter to obtain a clarified filtrate.
[0054] 2. Refining: the above-mentioned filtrate is washed with water, 10%, 20%, 30%, 40%, 50% and 75% concentration of methanol successively through the treated HP-20 macroporous adsorption resin, detected by HPLC, and collected rich Fractions containing parisin compounds were combined, concentrated under reduced pressure, and spray-dried to obtain 4.68 g of the plant extract. As determined by high performance liquid chromatography, the content of parisin compounds is 54.4%.
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