Supercharge Your Innovation With Domain-Expert AI Agents!

Psychrotrophs and application thereof in wastewater treatment

A sewage treatment, sewage biological treatment technology, applied in the low temperature resistant mixed bacteria, bacteria group and in the sewage treatment, sewage biological treatment field, can solve the problems of sludge bulking, reduction of treatment capacity, increase of operation cycle and operation cost, etc.

Inactive Publication Date: 2012-10-03
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Physiological treatment is the most commonly used sewage treatment method at present, but the biological treatment unit in the current biological treatment process is affected by the temperature due to the microbial activity, and its treatment capacity decreases with the decrease of temperature, resulting in a decline in the treatment efficiency and effect of the entire treatment process , sludge bulking and other phenomena appear
The winter climate in northern my country is cold and long, and the freezing period lasts for 3 to 6 months. Affected by climatic conditions, measures such as prolonging the residence time of sewage, reducing sludge load, increasing sludge return flow, and taking heat preservation or temperature rise for structures are generally adopted. To ensure that the effluent of sewage treatment in winter meets the standard, this will undoubtedly increase the operating cycle and operating costs, and the fundamental solution is to solve the adaptability of the microorganisms in the biological treatment unit to the temperature of the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Psychrotrophs and application thereof in wastewater treatment
  • Psychrotrophs and application thereof in wastewater treatment
  • Psychrotrophs and application thereof in wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Screening and Identification of Bacterial Strains

[0022] Of the three strains provided by the present invention, L126 is named Janthinobacterium lividum, L146 is named Flavobacterium hercynium, and L188 is named Pseudomonas gessardii. At present, the three strains have been deposited in the International Depository of Microorganisms of the Budapest Treaty: China Microorganism Culture Preservation Management Committee on General Microbiology Center (CGMCC). Address: Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, Zip code: 100101, date of deposit is March 18, 2009, and deposit numbers are CGMCC No 2956, CGMCC No 2957, and CGMCC No 2958.

[0023] Pyrotrophic bacteria L126, aerobic, Gram-negative, rod-shaped without spores, motile; the colony is white in the early stage, and turns purple in the later stage, with smooth, moist, round, and neat edges; growth temperature 4-35°C, 37°C No growth, the optimum growth t...

Embodiment 2

[0027] Embodiment two: the mensuration of growth curve of psychrotroph L126, L146, L188

[0028] Inoculate three strains of psychrotrophic bacteria L126, L146, and L188 in 5ml of complete liquid medium respectively, and culture them at 10°C and 180rpm for 24 hours, then inoculate 0.1ml of the bacterial suspension into 25ml of fresh complete liquid medium, under the same conditions Under culture, samples were taken at regular intervals to measure the absorbance value (OD) of the bacteria. 600 ), draw the growth curve, the results are shown in the attached figure 1 shown.

[0029] The results showed that the three strains could grow well under the low temperature condition of 10℃, but their growth rates were different; L188 had reached the maximum growth at 20 hours, while L146 grew slowly and had not reached the maximum growth at 42 hours. In mixed culture, different bacteria can play a role in different time periods.

Embodiment 3

[0030] Embodiment three: the influence of temperature on bacterial strain treatment effect

[0031] Inoculate three strains of psychrotrophic bacteria L126, L146, L188 and mesophilic strains Escherichia coli (E.coli) and Bacillus subtilis (S.subtilis) in complete liquid medium, place them at their respective optimum culture temperatures, and cultivate them at 180rpm After 20-24 hours, inoculate in simulated domestic sewage with 10% inoculum amount, incubate with 180rpm aeration, and measure the COD value change of simulated domestic sewage within 24 hours at 10°C, 15°C, 20°C, and 30°C respectively. The measurement results can be found in the appendix figure 2 shown.

[0032] The results showed that, compared with mesophilic bacteria Escherichia coli (E.coli) and Bacillus subtilis (S.subtilis), the degradation efficiency of psychrotrophic bacteria L126, L146 and L188 was significantly higher than that of mesophilic bacteria at low temperature; L126 and L146 , L188 strain has...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Psychrotrophs of Janthinobacterium lividum CGMCC No 2956, Flavobacterium hercynium CGMCC No 2957 and Pseudomonas gessardii CGMCC No2958 and application thereof in wastewater treatment. The preserved Psychrotrophs strains select the following complete mediums: 10 g of peptone, 5 g of yeastrel, 3 g of K2HPO4 and 1 g of glucose, the sterilization is carried out in 1000 ml of H2O with pH of 7.0 for 20 min at 121 DEG C, the Psychrotrophs strains after multiplication culture are inoculated in low-temperature wastewater in a separate or mixed manner, then cultured in an aerated manner at 10-30 DEG C and detected, and the action of the Psychrotrophs strains mixed in the low-temperature wastewater treatment is superior to that of the single strain. The Psychrotrophs strains are widely applied to the field of low-temperature wastewater treatment.

Description

field of invention [0001] The invention relates to the technical field of sewage biological treatment, in particular to the technical field of low-temperature-resistant mixed bacteria, bacterial groups and their application in sewage treatment. Background technique [0002] Water resources are an extremely important natural resource. The shortage and pollution of water resources have become important factors restricting the development of national economy and the construction of ecological environment. Therefore, the treatment and reuse of sewage is of great significance to improving the utilization of water resources, ensuring the development of the national economy and ecological construction. [0003] Physiological treatment is the most commonly used sewage treatment method at present, but the biological treatment unit in the current biological treatment process is affected by the temperature due to the microbial activity, and its treatment capacity decreases with the dec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/38C12R1/20
Inventor 茆军顾美英谢玉清房世杰
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com