Distribution method and distribution device of mobile phases of chromatographic columns

Abstract

The invention discloses a distribution method and a distribution device of mobile phases of chromatographic columns. The distribution method comprises the steps: adopting a circular distributor matched with a cross section for preparing a chromatographic column; divergently arranging distribution liquid paths of liquid phases outwards in a tree shape along the center on the distributor; installing a distribution hole on the end of each distribution liquid path of liquid phases, wherein the lengths from the center of the distributor to the same-specification distribution liquid paths of liquid phases of all the distribution holes are equal; evenly distributing the liquid phases from the center of the distributor along each distribution liquid path of liquid phases to each distribution hole; and then evenly distributing the liquid phases to fixed phases of chromatographic columns. The distribution method can effectively eliminate the defect that the residence times of the mobile phases on a distribution system have serious differences along with the increase of the diameters of the chromatographic columns, can realize the even initial distribution of the mobile phases entering the chromatographic columns and can not be influenced by the high-pressure transport of the mobile phases. The distribution device has the advantages of simple structural design, difficult blockage, easy washing and repeated use.

Description

technical field [0001] The invention belongs to the distributing technology of mobile phase in a chromatographic column for industrial preparation of chromatography, and in particular relates to a mobile phase distributing method and a distributing device especially suitable for a large-diameter chromatographic column. Background technique [0002] The main purpose of industrial preparative chromatography is to obtain pure substances from mixtures. In order to speed up the separation time and improve the separation efficiency, the amount of sample input to the preparative chromatographic device is large, resulting in a corresponding increase in the separation load of the preparative chromatographic column, and it is necessary to increase the column packing and increase the diameter and length of the preparative chromatographic column. , using a relatively large amount of mobile phase. After the liquid to be treated is injected into the chromatographic column, it moves in th...

AI Technical Summary

Problems solved by technology

But for the larger chromatographic column of diameter, especially the diameter of the chromatographic column of existing industrial preparation chromatogram increases gradually, the distribution uniformity of the fine distribution channel after entering the filter through a central inlet of the mobile phase on the distribution system will change with Decreases as the diameter of the column increases, eventually resulting in an uneven initial fluid distribution that affects the separation

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