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Small molecular peptide of specificity recognition cell surface integrin alphal3beta1

A cell surface, small molecule peptide technology, applied in the field of peptides containing 5-11 amino acids, can solve the problems of fluorescein transport, weak adhesion of small molecule peptides to cancer cells, etc.

Inactive Publication Date: 2010-02-03
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned small molecule peptides have weak ability to adhere to cancer cell A549, and cannot be used as a carrier of fluorescein to transport fluorescein into the tumor

Method used

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  • Small molecular peptide of specificity recognition cell surface integrin alphal3beta1
  • Small molecular peptide of specificity recognition cell surface integrin alphal3beta1
  • Small molecular peptide of specificity recognition cell surface integrin alphal3beta1

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Experimental program
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Embodiment Construction

[0018] In order to better understand the present invention, the technical effects of the present invention will be further clarified through the following examples and effect experiments.

[0019] 1. Preparation of small molecule peptides of the present invention

[0020] (1) Weigh 1g of NH on the surface 2 Resin beads of active groups, washed 3 times with dimethylamide, and then soaked in dimethylamide until the beads are fully swollen;

[0021] (2) Add cysteine ​​and N, N'-diisopropylcarbodiimide, react at room temperature for 2 hours, wash the beads 5 times with DMF, then add 20% piperidine, and react at room temperature for 5 minutes , then add 20% DMF, react for 15min, and completely remove the Fmoc protecting group;

[0022] (3) sequentially coupling aspartic acid, glycine, leucine, glycine, hydroxyproline, asparagine and cysteine ​​according to the method of step (2);

[0023] (4) Wash the beads connected with the last amino acid once with 25% trifluoroacetic acid an...

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Abstract

The invention provides a small molecular peptide of specificity recognition cell surface integrin alphal3beta1; the amino acid sequence of the small molecular peptide is cdGLGHypNc, wherein c expresses D type aminothiopropionic acid, d expresses D type aspartic acid, G expresses L type glycocoll, L expresses L type leucine, Hyp expresses L type 4-hydroxyproline, and N expresses L type aspartic acid. The small molecular peptide is obtained by the conventional solid-state reaction method, can specifically recognize the cell surface integrin alphal3beta1; therefore, the small molecular peptide can be used as image contrast agent such as a targeting carrier of the luciferin, transports the luciferin to the cancer cell surface of the high express alphal3beta1, realizes the molecule image diagnosis of tumor by a luciferin scanning device.

Description

technical field [0001] The invention relates to the field of organic chemistry, in particular to a peptide, especially a peptide containing 5-11 amino acids. Background technique [0002] Integrin is one of the members of the cell membrane surface glycoprotein receptor family, and it is an adhesion molecule that mediates cell-to-cell and cell-to-extracellular matrix (ECM). Integrin molecules are heterodimeric molecules composed of α and β subunits connected by non-covalent bonds, and participate in bidirectional signal transduction between cells and between cells and extracellular matrix, and play a vital role in the survival of cells. , Proliferation, differentiation, migration and invasion, adhesion and metastasis of cancer cells play an important regulatory role. Integrin molecules are widely distributed in vivo, different types of cells express different types of integrin molecules, and the expression of integrin molecules has obvious cell type characteristics, such as ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/04
CPCY02P20/55
Inventor 郭琳琅郭颖张帆李贵平
Owner SOUTHERN MEDICAL UNIVERSITY
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