Method for modifying DNA microfluidic chip micro-channel with mixed screening medium
A microfluidic chip and microchannel technology, applied in stress-stimulated microbial growth methods, DNA preparation, recombinant DNA technology, etc., to achieve the effects of simple and easy operation steps, short analysis time and long service life
Inactive Publication Date: 2012-07-04
EAST CHINA NORMAL UNIV
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Problems solved by technology
In the background technology, there is a method for modifying the microchannel of a DNA microfluidic chip with a single sieving medium, using a single sieving medium as a modifier, and when the microchannel is modified by this method for the analysis of DNA fragments, the microfluidic The adsorption of biochemical samples by the channel is greatly reduced, but the analysis time for biochemical sample DNA is longer, the analysis reliability is poor, and the service life of the modifier is shorter
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[0037] This embodiment operates completely according to the specific operation steps of the above-mentioned method. For the sake of brevity, only key technical data of each step are listed below.
[0038] In the first step, the addition of polyvinylpyrrolidone and hydroxyethylcellulose is 68mg, and the mass concentration of polyvinylpyrrolidone obtained and the mass concentration of hydroxyethylcellulose are 2.5%; in the third step, the The fluorescent dye is fluorescent cyanine monomer dye; in the fourth step, the volume of the medical syringe is 1mL.
[0039] The electrophoresis process of DNA sample analysis by DNA microfluidic chip modified in this example:
[0040] In this example, 0.5 μg / μL of ΦX 174-HaeIII digest DNA marker was taken and diluted 10 times with ultrapure water as a DNA sample.
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Abstract
A method for modifying a DNA microfluidic chip micro-channel with a mixed screening medium belongs to the technical field of DNA microfluidic chip electrophoretic analysis. and includes following steps: preparing a mixed screening medium of polyvinylpyrrolidone and hydroxyethyl cellulose as a modifier and modifying the DNA microfluidic chip micro-channel with the modifier. The method has easy operation. When the DNA microfluidic chip with the modified micro-channel is analyzed of DNA segments, the biochemical sample DNA screening effect is good; the analysis time is short and modifier servicelife is long. The invention is especially adapted to modifying the micro-channel of the DNA microfluidic chip to make the modified chip become the qualified and efficient DNA electrophoretic analysisimplement.
Description
technical field [0001] The invention relates to a method for modifying the microchannel of a DNA microfluidic chip with a mixed sieving medium, belonging to the technical field of DNA microfluidic chip electrophoresis analysis. Background technique [0002] With the rise of microfluidic chip analysis technology, DNA microfluidic chips are increasingly used in life sciences, and are increasingly used in the separation and sequencing of DNA fragments. DNA microfluidic chip analysis has the characteristics of fast analysis speed, low reagent consumption, and reusable chip. However, the adsorption of biochemical samples by the microchannel of the chip limits the general promotion of this technology to a certain extent. Therefore, on the basis of ensuring the reliability and repeatability of chip analysis, modifying the microchannels of DNA microfluidic chips has become one of the keys to popularize DNA microfluidic chip analysis technology and industrialization. In the backgro...
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IPC IPC(8): C12M1/42C12N15/10C12Q1/68G01N21/64
Inventor 李钢葛淑丽王清江何品刚方禹之
Owner EAST CHINA NORMAL UNIV