Pluripotent cells from rat and other species

A pluripotent cell and cell technology, which is applied in the field of maintaining the self-renewal ability of pluripotent cells, and can solve problems such as difficulty in guaranteeing the purity of cytokines
CN101657535AInactive Publication Date: 2010-02-24THE UNIV COURT OF THE UNIV OF EDINBURGH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
THE UNIV COURT OF THE UNIV OF EDINBURGH
Publication Date
2010-02-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

Pluripotent cells are derived and maintained in a self-renewing state in serum-free culture medium comprising a MEK inhibitor, a GSK3 inhibitor and an antagonist of an FGF receptor.
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Description

technical field

[0001] The present invention relates to maintaining the self-renewal capacity of pluripotent cells. The methods and compositions provided by the present invention are suitable for culturing and isolating pluripotent cells such as embryonic stem cells (ES), especially embryonic stem cells of mammals, including rats, mice, cattle, sheep, pigs and humans. In particular, the present invention relates to the self-renewal culture of rat, mouse and human pluripotent cells and methods and compositions related thereto. Background technique

[0002] It is well known that the initiation and maintenance of pluripotent stem cells in vitro are carried out in medium containing serum and leukemia inhibitory factor (LIF) (Smith et al. (1988) Nature 336:688-90). Such methods have been used to maintain pluripotent embryonic stem (ES) cells derived from "permissive" mice for many passages. The self-renewal of pluripotent stem cells can also be maintained in other ways, such as...

Claims

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