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Method for identifying falsification of distilled spirit by utilizing nucleic acid detection technique

A detection technology and liquor technology, which is applied in the field of authenticating liquor and can solve the problems of counterfeit and shoddy products disrupting the market order.

Inactive Publication Date: 2010-03-10
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

What it can do is to make a set of iron armor and helmets for the wine. When encountering cunning and sinister counterfeiters who adopt the method of stealing the beams and replacing the pillars, and the fake wine is packaged in real ways, this is just to add the anti-counterfeiting unit to the anti-counterfeiting theme. Above all, the anti-counterfeiting strategy based on the thinking of emphasizing the surface and not the inside will inevitably leave opportunities for counterfeiters, and eventually lead to counterfeit and shoddy products that disrupt the market order

Method used

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  • Method for identifying falsification of distilled spirit by utilizing nucleic acid detection technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Taking the addition of chicken genome DNA to liquor as an example to identify the authenticity of liquor

[0016] 1 According to the instructions of the blood / cell / tissue genomic DNA extraction kit (Tiangen Biochemical Technology Co., Ltd.), the genomic DNA in the chicken was extracted.

[0017] 2. Add 1mg chicken genomic DNA solution to 500mL liquor.

[0018] 3 Extraction of chicken genome DNA in liquor.

[0019] (1) Take 400 μL of white wine containing chicken genomic DNA and place it in a 1.5 mL centrifuge tube, add 40 μL of 3.0 mol / L sodium acetate solution, and mix well.

[0020] (2) Add 900 μL of ice-cold absolute ethanol, then add 14 μL of 1 mol / L magnesium chloride solution, and mix thoroughly. Then put it on ice for 1h.

[0021] (3) 0° C., 12000 r / min, centrifuge for 20 min, and recover chicken genome DNA.

[0022] (4) Carefully remove the supernatant with an automatic micropipette, and absorb all the droplets attached to the tube wall.

[0023]...

Embodiment 2

[0031] Example 2: Taking the addition of soybean genome DNA to liquor as an example to identify the authenticity of liquor

[0032] 1 According to the instructions of the New Plant Genomic DNA Extraction Kit (Tiangen Biochemical Technology Co., Ltd.), the soybean genomic DNA in fresh soybean leaves was extracted.

[0033] 2 Add 1 mg of soybean genomic DNA solution to 500 mL of liquor.

[0034] 3 Extraction of soybean genomic DNA in liquor.

[0035] (1) Take 400 μL of liquor containing soybean genomic DNA and place it in a 1.5 mL centrifuge tube, add 40 μL of 3.0 mol / L sodium acetate solution, and mix well.

[0036] (2) Add 900 μL of ice-cold absolute ethanol, then add 14 μL of 1 mol / L magnesium chloride solution, and mix thoroughly. Then put it on ice for 1h.

[0037] (3) 0° C., 12000 r / min, centrifuge for 20 min, and recover soybean genomic DNA.

[0038] (4) Carefully remove the supernatant with an automatic micropipette, and absorb all the droplets attached to the tube w...

Embodiment 3

[0047] Example 3: Taking the addition of yeast genome DNA to liquor as an example to identify the authenticity of liquor

[0048] 1 Extract the yeast genomic DNA according to the instructions of the Yeast Genomic DNA Extraction Kit (Tiangen Biochemical Technology Co., Ltd.).

[0049] 2 Add 10 μg of yeast genomic DNA solution to 500 mL of liquor.

[0050] 3. Extract the yeast genome DNA from liquor.

[0051] (1) Take 400 μL of liquor containing yeast genomic DNA and place it in a 1.5 mL centrifuge tube, add 40 μL of 3.0 mol / L sodium acetate solution, and mix well.

[0052] (2) Add 900 μL of ice-cold absolute ethanol, then add 14 μL of 1 mol / L magnesium chloride solution, and mix thoroughly. Then put it on ice for 1h.

[0053] (3) 0° C., 12000 r / min, centrifuge for 20 min, and recover yeast genomic DNA.

[0054] (4) Carefully remove the supernatant with an automatic micropipette, and absorb all the droplets attached to the tube wall.

[0055] (5) Add 700 μL of 70% ethanol, ...

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Abstract

The invention relates to a method for identifying falsification of distilled spirit by utilizing a nucleic acid detection technique, belonging to the biological high-technology field and comprising the following steps: adding a DNA solution to distilled spirit, and then extracting DNA added to the distilled spirit, adopting PCR to react so as to detect a target gene, and then judging the falsification of the distilled spirit according to the amount of amplified products in an electrophoresis result. The method has the characteristics of rapidness, simplicity, accuracy, strong specificity and high sensitivity. The method is suitable for providing technology support to each distilled spirit manufacturer, quality monitoring departments at various levels and market supervision departments to enable the manufacturers and the departments actively, rapidly and accurately take action in safeguarding legal rights and executing the law so as to protect the lawful rights and interests of enterprises and consumers.

Description

1. Technical field [0001] The invention relates to a method for identifying the authenticity of liquor by using nucleic acid detection technology, and belongs to the field of biological high technology. It has very important significance in liquor anti-counterfeiting. 2. Background technology [0002] Liquor is a unique traditional product in my country with a long history. In recent years, the demand for liquor, especially famous and high-quality products, has been increasing, and how to identify genuine and fake famous and high-quality liquor has been widely valued by people. At present, the anti-counterfeiting of liquor mainly adopts anti-counterfeiting packaging and anti-counterfeiting printing technology in the bottle mouth, bottle cap, outer packaging, logo, sealing, etc. of alcoholic products. Although these anti-counterfeiting measures play a certain role in anti-counterfeiting, they still have a certain distance from the psychological hope of the public and the ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N27/447
Inventor 杨德吉姚大伟郑新许家荣
Owner NANJING AGRICULTURAL UNIVERSITY
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