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Fast sieving method for alkaloid blood-pressure lowering matter mixed in Chinese medicinal herb blood-pressure lowering product

A technology of alkaloids and antihypertensive substances, which is applied in the direction of chemical analysis by precipitation, can solve the problems of complex components of Chinese herbal medicine products, unobvious reaction phenomena, and difficulty in screening, etc. The method is simple, low risk, and Responsive Effects

Active Publication Date: 2010-03-10
广东省药品检验所
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AI Technical Summary

Problems solved by technology

[0011] The above method can detect the precipitation of most alkaloids, but it is not feasible to directly detect the alkaloid antihypertensive substances in Chinese herbal antihypertensive products. First, because many Chinese herbal medicines contain alkaloids, it is easy to False positives occur, which makes it difficult to achieve the purpose of screening; more importantly, the ingredients of Chinese herbal medicine products are complex, and the alkaloids mixed with them are mostly mixed with preparation excipients. critical result

Method used

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  • Fast sieving method for alkaloid blood-pressure lowering matter mixed in Chinese medicinal herb blood-pressure lowering product

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0071] Kit composition:

[0072] Reagent 1: Ethanol-dichloromethane (V 乙醇 :V 二氯甲烷 =5:1);

[0073] Reagent 2: 0.05g / mL phosphomolybdic acid absolute ethanol solution.

[0074] The method of the present invention detects commercially available Qingnao Jiangya Tablets (batch number: 20081102):

[0075] Take 1 sample to be tested, crush it, add 3 mL of ethanol-dichloromethane (5:1), shake for 5 minutes, and filter; add 3 drops of phosphomolybdic acid ethanol solution with a concentration of 0.05 g / mL in the filtrate.

[0076] Result: No precipitation occurred and the solution was clear.

[0077] Standard method verification: Take 1 piece of sample to be tested, and use liquid-mass spectrometry to detect, the results show that reserpine, prazosin hydrochloride, atenolol and irbesartan are all negative.

example 2

[0079] Kit composition:

[0080] Reagent 1: Ethanol-dichloromethane (V 乙醇 :V 二氯甲烷 =5:1);

[0081] Reagent 2: 0.05g / mL phosphomolybdic acid absolute ethanol solution.

[0082] The method of the present invention detects commercially available Naoliqing Capsules (batch number: 080401):

[0083] Take 1 piece of sample to be tested, crush it, add 3 mL of ethanol-dichloromethane (5:1) and shake for 5 minutes, filter; add 3 drops of phosphomolybdic acid absolute ethanol solution with a concentration of 0.05 g / mL in the filtrate .

[0084] Result: yellow-white turbidity appears.

[0085] Standard method verification: Take 1 sample to be tested and detect it by liquid-mass spectrometry, and the result shows that it is positive for reserpine.

example 3

[0087] Kit composition:

[0088] Reagent 1: Ethanol-dichloromethane (V 乙醇 :V 二氯甲烷 =5:1);

[0089] Reagent 2: 0.05g / mL phosphomolybdic acid absolute ethanol solution.

[0090] The method of the present invention detects commercially available Qingnao Jiangya Tablets (batch number: 20081202):

[0091] Take 1 piece of sample to be tested, crush it, add 3 mL of ethanol-dichloromethane (5:1) and shake for 5 minutes, filter; add 3 drops of phosphomolybdic acid absolute ethanol solution with a concentration of 0.05 g / mL in the filtrate .

[0092] Result: cloudiness occurs.

[0093] Standard method verification: Take 1 piece of the sample to be tested and detect it by liquid-mass spectrometry, and the result shows that prazosin hydrochloride is positive.

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Abstract

The invention provides a fast sieving method for an alkaloid blood-pressure lowering matter mixed in a Chinese medicinal herb blood-pressure lowering product, which comprises the following specific steps of: firstly, extracting an alkaloid component from a sample by using an ethanol-dichloromethane solution (volume ratio of ethanol to dichloromethane is equal to 5:1), and then adding 0.05 g / ml molybdophosphoric aicd anhydrous ethanol solution. If sedimentation or turbidness appears, an alkaloid blood-pressure lowering matter is possibly doped in the sample. The method uses little commonly usedreagent to fast determine whether reserpine, minipress, atenolol and irbesartan are doped in the Chinese medicinal herb blood-pressure lowering product without any special instrument and is applicable to preliminarily sieve the Chinese medicinal herb blood-pressure lowering product illegally doped with the alkaloid blood-pressure lowering matter on site (a pharmaceutical factory or a drug store),thereby not only saving a plurality of human and material resources, but also greatly lowering omission factor of the conventional casual inspection.

Description

technical field [0001] The invention relates to a method for analyzing materials by virtue of their chemical properties, in particular to a detection method for alkaloid antihypertensive drugs. Background technique [0002] Cardiovascular disease, a common cardiovascular disease with a history of hypertension, has become a major cause of death and disability and seriously endangers people's health. Drugs for the treatment of high blood pressure include Western medicine and Chinese patent medicine. Among them, western medicine works quickly and has obvious antihypertensive effect, but has serious side effects when taken for a long time; while natural Chinese medicines made of Chinese herbal antihypertensive products (including Chinese patent medicines, health products and food made of Chinese herbal medicines) have less side effects, but Onset is slow. In order to cater to the mentality of hypertensive patients who want to reduce blood pressure quickly and have less side ef...

Claims

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Application Information

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IPC IPC(8): G01N31/02
Inventor 李泳雪莫结丽肖树雄贾宜军
Owner 广东省药品检验所
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