Method for continuously extracting and separating 1-deoxynojirimycin (DNJ) and flavone from folium mori

Abstract

The invention relates to a method for continuously extracting and separating 1-deoxynojirimycin (DNJ) and flavone from folium mori; the key points are that: folium mori and ethanol are mixed, and extracted and filtered centrifugally, and then flocculating agents are added for flocculation and precipitation after being concentrated; the precipitation is removed, and the filterate is added in a plurality of cation exchange resin columns which are communicated, and flows through a macroporous absorption resin column connected with the resin columns in series; after the filtrate adding is finished, the filtrate is washed until outflow liquid from the macroporous absorption resin column is colourless, and then the macroporous absorption resin column is disconnected, and then the 1-deoxynojirimycin (DNJ) is eluated with ammonia water or ammoniac ethanol solution from the cation exchange resin columns; the eluate is eluated by the cation exchange resin columns again, the operation is repeated, and then the eluate is collected, is concentrated and filtered with a 80D nanofiltration membrane and then is spray-dried, so as to obtain claybank powdery 1-deoxynojirimycin (DNJ). 3-10 percent ofalcohol is used for washing the macroporous absorption resin column to remove colloidal impurities, and then 50-70 percent of alcohol is used for washing flavone, the claybank eluate is collected, decompression and concentration are carried out to recover alcohol, and then spray-drying is carried out to obtain the claybank powdery folium mori general flavone.

Description

technical field [0001] The invention relates to a method for continuously extracting and separating 1-deoxynojirimycin (DNJ) and flavonoids from mulberry leaves. Background technique [0002] Mulberry leaf is the dry leaf of Moraceae plant mulberry, which is cultivated or wild, and has strong adaptability to soil, and it is mostly produced in most parts of the country. Bitter in the mouth, sweet, cold in nature. Studies have found that mulberry leaves have anti-inflammatory and hypoglycemic effects pharmacologically, and can lower blood fat and diuresis. Mulberry leaves have the above activities and pharmacological effects, mainly because their components contain 1-deoxynojirimycin (DNJ), flavonoids and other components, so it is very necessary to extract them from mulberry leaves for concentrated use . [0003] 1-Deoxynojirimycin (DNJ) is a strong α-glucosidase inhibitor, which inhibits α-glucosidase in the small intestine to decompose disaccharides such as sucrose and m...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that a high-speed centrifuge is used for material-liquid separation, which is expensive in industry; in addition, the material liquid is separated from flavonoids through a macroporous resin, and then the alkaloids are extracted through a cationic resin, which is easy to lose precious and relatively low content. Low active ingredient 1-deoxynonomycin (DNJ) (0.2%-0.4% in mulberry leaves)

But its disadvantage is that this process uses organic solvents such as chloroform and n-hexane when preparing alkaloids, which are toxic and difficult to remove from the product, and are not suitable for large-scale industrial production

The advantage is that the separated product can be easily obtained, but the disadvantage is that the production step needs to cool the extract to 10°C to 30°C, the conditions are relatively harsh, and methanol, diethylamine, triethylamine, etc. are used in the process Toxic and flammable organic solvents are not conducive to clean production