A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and DNA markers for selecting the plant of said raphanus sativus line

A technology of DNA markers and radish, applied in botany equipment and methods, angiosperms/flowering plants, plant products, etc., can solve the problems of limited sample analysis and high cost of markers

Active Publication Date: 2010-03-24
DONGBU HITEK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these markers are limited by sample analysis and require high costs

Method used

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  • A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and DNA markers for selecting the plant of said raphanus sativus line
  • A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and DNA markers for selecting the plant of said raphanus sativus line
  • A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and DNA markers for selecting the plant of said raphanus sativus line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] Example 1: Development of a new type of D-CGMS radish line and research on its characteristics

[0120] Breeding of new D-CGMS

[0121] The inventor has collected novel male sterility resources in order to improve F 1 Production capacity of hybrid seeds. Then, the present inventors studied organ tissues and pollen development of flowers of 20 plants of the radish line collected from Uzbekistan and the like distributed at the National Institute of Horticulture in Korea.

[0122] As a result, a line exhibiting male sterility (nucleoplasmic sterility: CGMS) by inhibiting pollen development was selected and named "D-CGMS (radish variety D-CGMS)".

[0123] The flowers and stamens of selected D-CGMS, normal fertile lines and Ogura radish line (Ogura-CGMS) were observed under a dissecting microscope. Ogura-CGMS is generally used to produce hybrid seeds, so it was used here as a control.

[0124] As a result, pollen development was very weak in the novel male sterile line ...

Embodiment 2

[0136] Example 2: Induction of D-CGMS radish male sterility and production of F 1 Proof of Generation Hybrid Seed

[0137] The D-CGMS radish line plant selected from Example 1 is used as the female parent to cross the male fertile line plant to produce the CGMS radish line F with nucleoplasmic interaction sterile type 1 generation hybrid seeds. To compare the CGMS induction rate of the D-CGMS radish line with that of the Ogura-CGMS radish line, male fertile plants for crossing with the D-CGMS radish line were crossed with Ogura-CGMS as the female parent. Pollen development and viability were studied to confirm that the F 1 Whether male sterility is introduced in hybrid seeds. Male fertile line plants (male line) are selected from lines isolated from "JungSangYeoreum" (DB002), HannongYeoreum (DB003), lines isolated from "HannongYeoreum" (DB111), SamYang (DB021), DeaBuRyeongYeoreum (DB084), Lines isolated from "HaChu" (DB092), and plants of those male-fertile lines collected...

Embodiment 3

[0142] Example 3: Development of male fertility restoration gene resources of D-CGMS radish lines

[0143] In order to screen male fertile restorer lines selected from the D-CGMS radish line in Example 1, 10 male fertile lines collected from Uzbekistan and Russia were crossed with D-CGMS radish line plants as female parents. Those 10 male-fertile lines as male lines were collected from foreign countries (Uzbekistan and Russia), so the names of their variants are unknown, so only the names of their lines are given in this paper. To study in F 1 Pollen development and vigor were studied to determine whether male sterility was introduced in the first generation hybrid seeds.

[0144] As a result, among the 10 combinations with male-fertile lines, two hybrid combinations confirmed the emergence of male-fertile offspring. In the F produced by the hybridization of D-CGMS and DB112 1 Male sterile and male fertile progenies were segregated from hybrid seeds of the first generation,...

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Abstract

The present invention relates to a method for producing hybrid seeds using a novel Cytoplasmic-Genic Male Sterility (CGMS) Raphanus sativus line plant and DNA markers for the selection of the same, more precisely a novel CGMS (D-CGMS) Raphanus sativus line plant, a method for producing hybrid seeds using the same, chloroplast DNA-based markers for the selection of the D-CGMS Raphanus sativus lineplant having the nucleotide sequence represented by SEQ. ID. NO: 5, mitochondrial DNA-based SNP (Single Nucleotide Polymorphism) markers for the selection of the D-CGMS Raphanus sativus line plant located on the 171st nucleotide of the nucleotide sequence represented by SEQ. ID. NO: 12. The D-CGMS Raphanus sativus line plant of the present invention can be effectively used for the production of hybrid seeds owing to its high stability of male sterility. The chloroplast DNA-based markers and mitochondrial DNA-based SNP markers for the selection of D- CGMS Raphanus sativus line plants can be very effective in the selection of D-CGMS Raphanus sativus line plants during breeding process.

Description

technical field [0001] The present invention relates to the method that utilizes nucleoplasmic interaction male sterile type (CGMS) radish line plant (Raphanus sativus line plant) and the DNA mark that is used for selection and breeding of this plant to produce hybrid seed, specifically, the present invention relates to novel CGMS ( D-CGMS) radish plants, using the radish plants, breeding D-CGMS chloroplast DNA markers of radish plants, which have the nucleotide sequence shown in SEQ.ID.NO: 5, and breeding D-CGMS A single nucleotide polymorphism (SNP) marker based on mitochondrial DNA of radish plants, wherein the SNP marker is located at the 171st residue of the nucleotide sequence shown in SEQ.ID.NO: 12, and a method for producing hybrid seeds. Background technique [0002] In the field of plant breeding, F 1 Generations of hybrid breeding have continued to exploit heterosis. Most vegetable seeds on the market are F 1 generation hybrid seeds. f 1 The seed production m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29
CPCA01H1/02A01H5/10C12Q1/6888A01H5/00C12Q1/6886C12Q2600/13
Inventor 李永杓成淳基金成吉安荣舜金孝贞林采完
Owner DONGBU HITEK CO LTD
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