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Whole blood assay

A technology for whole blood samples and measured values, applied in the field of whole blood detection, can solve the problems of increasing the complexity of system design and increasing costs

Inactive Publication Date: 2010-03-24
QUOTIENT DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For point-of-care, medical offices, or clinics, small volumes of whole blood samples can be separated from plasma components by filtration or other mechanical manipulations, which add complexity to the system design and thus increase cost

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0053] A known volume (eg, 2.0 ml) of reagent containing buffer and a mixture of enzyme and reactants for the enzyme-linked reaction (shown below) is added to the reaction cuvette.

[0054]

[0055]

[0056]

[0057] The mixture was added to a photometer and excited at 510 nm. Blank transmittance readings were taken at <1 second intervals for approximately 5 seconds.

[0058] Then, at t 0 At time points, a fixed volume (5 μL) of blood, whose plasma cholesterol level is known, is added to the cuvette and mixed. The transmittance was measured at 510 nm when the mixing was complete and the reaction time was measured for 3 minutes. The time taken to create mixing and stop the vortex in the liquid means the transmittance recorded after the 10th second from the addition of the blood sample.

[0059] Table 1 below lists examples of blank and blood transmittance signals and reference readings.

[0060] Table 1

[0061] reading time

Transmittance

0....

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Abstract

A method and apparatus to estimate the concentration of a target substance (e.g. Cholesterol or CRP) in the plasma component of awhole blood sample without the need to separate the red blood cells from the plasma prior to testing,thereby simplifying the design and construction of the test device. The invention achieves this by measuring the analyte under investigation in a time dependent (bio- / immuno-) chemical reaction and measuring separately, a marker substance (e.g haemoglobin) for the estimation of red blood cell volume, using a non-time-dependent alteration in physical property of the reaction mixture (in this instance, transmission) attributed to inherent filter effects on sample addition. These non-time- dependent changes are not part of the reaction chemistry, and are resolved from the time dependent alteration in physical property caused by the assay chemistry by continuous measurement and mathematical modelling. Algorithms that combine these two parameters are used to estimate the target substance and compensate for variations in the percentage haematocrit of the sample. The method equalises the assay response for subtle variations in patient sample (e.g. haematocrit).

Description

technical field [0001] The invention relates to a method for detecting substances in whole blood samples. Background technique [0002] UK patent application 0606450.5 describes the detection of glycated proteins by fluorescence quenching, in which a method is used to distinguish between time-independent changes due to intrinsic filtering and time-dependent changes due to analytical chemicals and equipment to measure the concentration of non-fluorescent substances (eg, hemoglobin) (Measurement of Hemoglobin-A1c). This approach avoids the requirement to perform separate photometric and other assays, thereby simplifying the detection method and associated instrumentation. [0003] The present invention is a modification of this method for measuring other substances, without the use of a fluorometer, by continuously monitoring and using the developing signal (e.g., transmittance) to measure not only the reacting substance, but also the Compensate for the initial amount of sam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/487G01N33/49G01N33/92G01N33/96
CPCG01N33/92G01N33/49G01N2333/904G01N33/72G01N2333/918
Inventor 约翰·菲利普·瓦塞安德利安·理查德·格雷大卫·皮尔斯沃
Owner QUOTIENT DIAGNOSTICS
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