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A starfish extract and its application in the preparation of antitumor drugs

An anti-tumor drug and extract technology, applied in the field of starfish extract and its application in the preparation of anti-tumor drugs, can solve the problems of shellfish farming hazards and other problems, and achieve low price, safe and reliable ingredients, and simple preparation methods Effect

Active Publication Date: 2011-12-28
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its reproductive ability and regeneration ability are very strong, and it has great harm to the shellfish farming industry.

Method used

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  • A starfish extract and its application in the preparation of antitumor drugs
  • A starfish extract and its application in the preparation of antitumor drugs
  • A starfish extract and its application in the preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of Starfish Extract

[0027] In this embodiment, the Feibai maple starfish Archaster typicus is used as a raw material to prepare a starfish extract, which specifically includes the following steps:

[0028] (1) Take 5 kg dry weight of Feibai maple sea star Archaster typicus, extract 4 times with 5 liters of ethanol at room temperature, combine the obtained extracts, and concentrate the extracts at low pressure to dryness to obtain 170 g of ethanol extract;

[0029] (2) Suspend the above-mentioned ethanol extract in 5 liters of water, then add 5 liters of chloroform for liquid separation extraction, and collect the chloroform layer extract and the water layer extract respectively;

[0030] (3) The chloroform layer extract obtained in step (2) is concentrated to obtain 100 grams of extract, which is a starfish chloroform layer extract;

[0031] (4) The water layer extract obtained in step (2) is passed through a macroporous adsorption resin, and then the resi...

Embodiment 2

[0037] Example 2 In vitro anti-tumor experiment of starfish extract

[0038] In this example, three tumor cell lines, Colo205 (human colon cancer cells), NCI-H446 (human small cell lung cancer cells) and MDA-MB-435 (human breast cancer cells) were selected, and the starfish extract prepared in Example 1 (Sea star trichloromethane layer extract and sea star water layer extract) MTT test was performed to detect the anti-tumor activity of the starfish extract prepared in Example 1; Colo205 (human colon cancer cells), NCI-H446 (human small cell lung cancer) Cells) and MDA-MB-435 (human breast cancer cells) are commercially available.

[0039] The present invention is divided into the following three groups:

[0040] (1) Blank control group;

[0041] (2) Trichloromethane experimental group: In step (3) of Example 1, the extract obtained by trichloromethane extraction;

[0042] (3) Water layer experiment group: In step (4) of Example 1, the extract obtained by water layer extraction.

[0043...

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Abstract

The invention discloses a starfish extract and its application in the preparation of antitumor drugs. The starfish extract is obtained by extracting the maple starfish with an organic solvent, collecting the extract, filtering, concentrating and drying the extract; After the substance was suspended in water, chloroform was added for liquid separation and extraction; the chloroform layer was collected, concentrated and evaporated to dryness to obtain starfish chloroform layer extract; the water layer was collected, adsorbed by a macroporous adsorption resin, and the resin was eluted with water to Colorless or light yellow, then elute with ethanol-water solution, collect the eluate, concentrate and dry the eluate to obtain the starfish water layer extract. The starfish extract of the invention has medium cell activity and can be used for preparing antitumor drugs, such as anti-colon cancer drugs, anti-lung cancer drugs or anti-breast cancer drugs.

Description

Technical field [0001] The invention relates to the technical field of organic compounds, in particular to organic compounds derived from marine organisms, in particular to a starfish extract and its application in the preparation of antitumor drugs. Background technique [0002] Starfish (starfish) was first published in "Chinese Medicinal Animals", and belong to the Echinodermata (Echinodermata) starfish (Asteroidea) animal. There are many types and rich resources. There are 5 orders and nearly 1,500 kinds. There are about 100 kinds in our country. They are distributed in the intertidal zone to 600m deep water. Its appearance has three arms, four arms, more than ten arms, and as many as fifty arms. Starfish is a marine benthic animal with a cruel nature. It specializes in clams, oysters, scallops, abalones, sea cucumbers, etc. Its reproductive capacity and regeneration capacity are very strong, which has great harm to the shellfish breeding industry. [0003] Starfish tastes s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/56A61P35/00
Inventor 杨献文董光闻克威周雪峰徐暾海刘永宏
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI