Method for preparing nano tricalcium phosphate silk protein complex three-dimensional porous material

A three-dimensional porous, tricalcium phosphate technology for application in the field of medical materials

Inactive Publication Date: 2010-05-12
ZHONGSHAN HOSPITAL FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the use of silk fibroin in combination with β-TCP

Method used

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  • Method for preparing nano tricalcium phosphate silk protein complex three-dimensional porous material
  • Method for preparing nano tricalcium phosphate silk protein complex three-dimensional porous material
  • Method for preparing nano tricalcium phosphate silk protein complex three-dimensional porous material

Examples

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Effect test

Embodiment 1

[0014] A method for preparing a composite three-dimensional porous bone tissue engineering scaffold. The specific steps are: adding β-tricalcium phosphate with a particle size of 100-300nm to 1ml of n-butanol at a mass volume ratio of 3.6g:100ml, and mechanically rotating at 500rpm. Stir and carry out ultrasonic dispersion at the same time. The ultrasonic power is 180W and the frequency is 50KHz; The mass ratio of the mixture is 10:100, mechanically stirred at 200rpm for 1min; adding sodium chloride particles with a particle size of 350-500μm as a pore-forming agent, adding sodium chloride particles with a mass of 24g, placing them at room temperature for 24 hours, adding Soak in water for 24 hours to elute the pore-forming agent. The weight ratio of sodium chloride particles to water is 1g:40ml; after the pore-forming agent is washed, freeze-dry to obtain a composite three-dimensional porous bone tissue engineering scaffold.

[0015] The porous composite scaffold prepared by ...

Embodiment 2

[0017] A method for preparing a composite three-dimensional porous bone tissue engineering scaffold. The specific steps are: adding β-tricalcium phosphate with a particle size of 100-300nm to 1ml of n-butanol at a mass-volume ratio of 30:100, and mechanically stirring at a speed of 500rpm At the same time, ultrasonic dispersion was carried out, the ultrasonic power was 180W, and the frequency was 50KHz; after 5 minutes, the above dispersion liquid was added dropwise to 6ml of silk fibroin solution with a mass fraction of 10%, to control the separation of β-tricalcium phosphate and silk fibroin. The mass ratio is 50:100, mechanically stirred at 200 rpm for 1 min; adding sodium chloride particles with a particle size of 350-500 μm as a pore-forming agent, adding 24 grams of sodium chloride particles, and placing them at room temperature for 24 hours. Add water and soak for 24 hours to elute the pore-forming agent. The weight ratio of sodium chloride particles to water is 1g:40ml;...

Embodiment 3

[0020] A method for preparing a composite three-dimensional porous bone tissue engineering scaffold. The specific steps are: adding β-tricalcium phosphate with a particle size of 100-300nm in a mass-to-volume ratio of 24g:100ml into 1ml of n-butanol, mechanically stirring at a speed of 500rpm and At the same time, carry out ultrasonic dispersion, the ultrasonic power is 180W, and the frequency is 50KHz; after 5 minutes, the above dispersion liquid is added dropwise to 6ml of silk fibroin solution with a mass fraction of 8%, to control the quality of β-tricalcium phosphate and silk fibroin The ratio is 30:100, mechanical stirring at 200rpm speed for 1min; add sodium chloride particles with a particle size of 350-500μm as a pore-forming agent, the quality of the added sodium chloride particles is 24g, and after 24 hours at room temperature, add water Soak for 24 hours to elute the pore-forming agent. The weight ratio of sodium chloride particles to water is 1g:40ml; after the por...

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Abstract

The invention provides a method for preparing a complex three-dimensional porous bone tissue engineering scaffold, which is characterized by specifically comprising the following steps: adding beta-tricalcium phosphate into n-butyl alcohol, mechanically stirring and ultrasonically dispersing the mixture at the same time, gradually dropping the dispersed solution into 6 to 10 mass percent silk fibroin solution, controlling the mass ratio of the beta-tricalcium phosphate to the silk fibroin at 10-50:100, and mechanically stirring the solution; adding sodium chloride granules with the grain diameter of 350 to 500 mu m serving as a pore-forming agent into the solution, placing the solution for 24 hours at the room temperature, and adding water into the solution and soaking the granules for 24 hours to wash out the pore-forming agent; and freezing and drying the mixture after the pore-forming agent is washed out to obtain the complex three-dimensional porous bone tissue engineering scaffold. The complex three-dimensional porous bone tissue engineering scaffold is characterized in that: the porosity of the scaffold is 92 to 98 percent, the aperture is 300 to 450 mu m, the compression modulus is 5.75 to 13.43MPa, and the water absorption rate is as high as 3,300 percent and the like; and the scaffold is suitable for restoration and replacement of bone defects.

Description

technical field [0001] The invention relates to a preparation method of a composite three-dimensional porous bone tissue engineering scaffold, which is used for repairing and replacing bone defects and belongs to the technical field of medical materials. Background technique [0002] Bone defect has always been a difficult point in orthopedic clinic. In recent years, the development of tissue engineering technology has brought new hope for its treatment. Scaffold materials are an important part of bone tissue engineering research. The ideal scaffold material should have good biocompatibility; 2) biodegradability; 3) osteoinductive and osteoconductive capabilities; 4) porosity, and to ensure appropriate pore size and porosity for sufficient cells Provide space for the survival of cells and the migration of tissue cells. [0003] β-calcium phosphate (β-TCP) is the main component of the inorganic phase in human hard tissues, has excellent biological effects, and can guide th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/12A61L27/56
Inventor 董健周健文剑川方涛林邵正中
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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