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Method for preparing blumea riparia water-soluble polysaccharides

A technology of water-soluble polysaccharide and gui aina incense, applied in the field of medicine, can solve the problems of limited research and rare diangui aina incense, etc., and achieve the effects of simple process flow, significant hemostatic activity, and energy saving.

Inactive Publication Date: 2010-05-19
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few research reports on the pharmacological activity of the specific chemical components of Ainaxiang yunnanensis at home and abroad.
In particular, the relevant research on the polysaccharides contained in the Ainaxiang plant is still very limited.

Method used

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  • Method for preparing blumea riparia water-soluble polysaccharides
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  • Method for preparing blumea riparia water-soluble polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The first preparation method of Yunnan laurel Aina perfume soluble polysaccharide, the operation steps are as follows:

[0046] 1. Extraction: add 3.0 kg of dried medicinal materials of Aina cinnamon from Diangui Aina to each time add 15 times the weight of deionized water and decoct twice for 3 hours each time, filter and combine the filtrates.

[0047] 2. Membrane separation: use membrane separation equipment for rough separation, and the filtrate extracted in step 1 has a molecular weight cut-off of 10 5 Da ceramic membrane, molecular weight less than 10 5 The filtrate of Da passes through a molecular weight cut-off of 10 4 Da organic membrane, the filtrate continues to pass through the molecular weight cut-off of 10 3 Da organic membranes to obtain molecular weights in the range of 10 3 to 10 4 The retentate was concentrated under reduced pressure to 500 mL.

[0048] (3) Deproteinization: Add about 170mL of trichloroacetic acid aqueous solution with a weight co...

Embodiment 2

[0053] The second preparation method of Diangui Aina perfume soluble polysaccharide, the operation steps are as follows:

[0054] 1. Extraction: Add 3.0kg of dried medicinal herbs of Aina osmanthus Dianensis into 10 times the weight of distilled water and decoct 3 times for 2 hours each time, filter and combine the filtrates.

[0055] 2. Membrane separation: use membrane separation equipment for rough separation, and the filtrate extracted in step 1 has a molecular weight cut-off of 10 5 Da ceramic membrane, molecular weight less than 10 5 The filtrate of Da passes through a molecular weight cut-off of 10 4 Da organic membrane, the filtrate continues to pass through the molecular weight cut-off of 10 3 Da organic membranes to obtain molecular weights in the range of 10 3 to 10 4 The retentate was concentrated under reduced pressure to 500 mL.

[0056] 3. Deproteinization: Under ice bath conditions, add about 170mL of 20% trichloroacetic acid aqueous solution while stirrin...

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Abstract

The invention discloses a method for preparing blumea riparia water-soluble polysaccharides. One method of the invention comprises the steps of extraction, film separation, protein removal, desalination, decoloration, refining and refining. The second method of the invention comprises the steps of extraction, film separation, protein removal, silica gel column chromatographic separation, decoloration, dialysis and refining. The method has the advantages of simple process flow, energy conservation, high product purity, stable quality and the like. The production of the invention particularly has significant hemostatic activity in blood coagulation recalcification tests and isolated uterine tests of mice.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for preparing a perfume-soluble polysaccharide from Diangui Aina. Background technique [0002] Diangui Ainaxiang is a plant of the genus Ainaxiang in the family Asteraceae. It is recorded in "Chinese Medicine Sea" that Diangui Ainaxiang has a meridian distribution of nature and flavor: light, sweet and flat, enters the liver, kidney, and bladder; efficacy and indications: blood circulation Hemostasis - This product is sweet and fades into the liver, nourishing the liver and promoting blood circulation, astringent, astringent and hemostasis, has the function of promoting blood circulation without hurting blood, and stopping bleeding without leaving stasis. Hemostatic function, can be used for early menstruation, postpartum bleeding and other gynecological bleeding symptoms. [0003] Diangui Ainaxiang has a long history as a folk medicine in Guangxi. It is taken as a de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P7/04A61P15/00A61K36/28
Inventor 林翠梧廖敏富黄丽许子竞
Owner GUANGXI UNIV
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