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Model cell chip, apparatus for evaluating drug effect using the model cell chip and method of evaluating drug effect

A model cell and drug efficacy evaluation technology, applied in tissue cell/virus culture devices, measurement devices, biochemical cleaning devices, etc., can solve problems such as data dispersion, information buried in groups, and attribute cell blocks cannot be obtained

Inactive Publication Date: 2010-05-19
NAT UNIV CORP TOKYO MEDICAL & DENTAL UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, in this case, unlike cultured cells, it is not possible to obtain a cell mass with uniform properties throughout
Therefore, it leads to the problem that the resulting data is scattered or the information is buried in the cluster

Method used

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  • Model cell chip, apparatus for evaluating drug effect using the model cell chip and method of evaluating drug effect
  • Model cell chip, apparatus for evaluating drug effect using the model cell chip and method of evaluating drug effect
  • Model cell chip, apparatus for evaluating drug effect using the model cell chip and method of evaluating drug effect

Examples

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Embodiment Construction

[0193] figure 1 It is a perspective view schematically showing an example of the structure of the model cell chip and the drug efficacy evaluation device using the model cell chip according to the first embodiment of the present invention. figure 2 is a schematic representation figure 1 A perspective view showing an example of the structure of the model cell chip and the cell holding unit CH of the drug efficacy evaluation device using the model cell chip.

[0194] 100 is a drug efficacy evaluation device using a model cell chip, which is mainly composed of components built on a transparent substrate 1 . The transparent substrate 1 is an optically transparent material, such as a glass substrate or a silicon substrate. 2 is a micro electrode, which is set as a transparent electrode made of ITO, for example, and is arranged on the transparent substrate 1 . 2' is the lead-out line of the tiny electrode 2. 3 1 、3 2 、3 3 and 3 4 It is an agarose gel wall, and there is a ...

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Abstract

The present invention provides an apparatus for evaluating a drug effect enabling on-chip evaluation of the effect of a drug while the drug is acting on hERG-expressing cells. The present invention also provides a myocardial toxicity test apparatus and method therefor enabling in vitro myocardial toxicity testing that has previously been performed in vivo. A pulsating cell population and hERG-expressing cells (target model cells) are suitably isolated and arranged on a transparent substrate so that the two form gap junctions. The hERG-expressing cells are arranged on transparent electrodes provided on the transparent substrate. The hERG-expressing cells are exposed to a flow of a liquid containing a drug such that the drug acts thereon. The difference between the normal pulsation of hERG-expressing cells and the pulsation when a drug is acting thereon is captured via electric signals obtained from electrodes, and the properties of the change in potential are evaluated.

Description

technical field [0001] The invention relates to a model cell chip, a drug efficacy evaluation device using the model cell chip and a drug effect evaluation method. In particular, the present invention relates to a model cell chip using hERG-expressing cells, a drug efficacy evaluation device using the model cell chip, a device for testing cardiomyocyte toxicity of drugs, a chip for testing cardiotoxicity, and a method for testing cardiotoxicity. Background technique [0002] Bioassays are often used to observe changes in the state of cells, their response to pharmaceutical agents, and the like. In conventional bioassays, cultured cells are generally used in many cases. In this system, a plurality of cells are used for measurement, so the average value of the value of the cell population is observed as if observing the properties of a single cell. [0003] However, in practice, cells with synchronized cell cycles in a population are rare, and individual cells express protei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34C12M5/06C12Q1/06G01N27/28G01N27/30G01N27/327G01N27/416
CPCG01N33/54366C12N2503/02G01N33/5061G01N33/4836
Inventor 安田贤二杉山笃安东贤太郎野村典正寺薗英之金子智行福岛守
Owner NAT UNIV CORP TOKYO MEDICAL & DENTAL UNIV
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