Method for fixing mineral leaching rod-shaped bacteria for AFM analysis under strong acid condition

Abstract

The invention discloses a method for fixing mineral leaching rod-shaped bacteria for AFM analysis under a strong acid condition. In the method, a filter membrane (mixed cellulose ester millpore filter membrane) with an aperture of 0.22 micron is used as a fixing material, a domestic filter (the diameter is 25 millimeters) is used as a filter device, and a disposable medicine injection is utilized for filtration through pressurization. In a preparation process of fixing the mineral leaching bacteria, the filter condition and the filter device have small influences on the activity of the bacteria, no toxicity and convenient operation. In a solution with a pH value of 2, the prepared bacteria have low drift rate, good fixing effect and higher stability, thus an atomic force microscope (AFM) is suitable for analyzing the mineral leaching bacteria under the strong acid condition. The method can be widely applied to the field of AFM researches and has good application prospect and practical values.

Description

technical field [0001] The invention relates to a method for fixing microorganisms, in particular to a method for fixing rod-shaped bacteria used for AFM analysis under strong acid conditions. Background technique [0002] Atomic force microscopy (AFM) has atomic-level resolution and can detect under vacuum, atmosphere, liquid and temperature-controlled conditions. Samples can be conductive, non-conductive, flexible, or even living biological samples. It can characterize the surface morphology of samples, study the surface properties of samples, and probe the kinetic process of intermolecular interactions under near-physiological conditions, so AFM has attracted widespread attention. [0003] AFM mainly interacts with the atoms on the sample surface through the probes on the microcantilever. However, in the air environment, since water vapor (or other gas molecules) forms a hydration film (or other adsorption film) on the surface of the needle tip and the sample, when the n...

AI Technical Summary

Problems solved by technology

Unfortunately, the imaging effect is still far from ideal

This is mainly because the surface of the cell membrane is relatively soft, and the fixation with the substrate is weak. The pressure of the probe will greatly increase the contact area between the probe and the cell surface, thereby damaging the cell or causing the cell to drift.

And these method steps are loaded down with trivial details, have increased the expenditure of operation difficulty and expense

There are also experts who use microporous membranes to fix round biological cells, but there is no report on the use of membranes to fix rod-shaped bacteria at home and abroad, and most of the leaching bacteria are rod-shaped

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