Method for relieving stress response in manual operations of juvenile fish of sebastes schlegeli hilgendorf

A technology of stress response and manual operation, applied in application, fish farming, climate change adaptation, etc., can solve problems such as surface injury of fish, physiological dysfunction of aquatic animals, redness and swelling of wounds, etc.

Inactive Publication Date: 2010-06-09
MARICULTURE INST OF SHANDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this fish is lively by nature and is highly sensitive to external stimuli; it struggles and jumps strongly after leaving the water, and there are sharp spines on the dorsal fin, anal fin, and gill cover, which are very easy to stab each other, and it is difficult for handling, transportation, individual marking, Manual operations such as immunization injections and physiological experiments have brought great difficulties
The above-mentioned operations are mostly carried out directly. The stress response of the flat scorpionfish not only makes various operations very inconvenient, but also causes surface injuries to the fish and induces diseases. redness, stinging
[0003] In addition, the stress response will inhibit the immune function of aquatic animals. Aquatic animals that are often in a state of stress have physiological dysfunction and are more likely to be infected with diseases.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In August 2009, in the seed cultivation workshop of Yantai Baijia Aquatic Products Co., Ltd. located in Huangshanguan Town, Longkou City, Yantai, the water temperature was 25-26°C, salinity 29-30, pH 8.0-8.2, DO>6mg / L The seawater, with a 20L plastic storage box as the container, the volume of seawater is 10L, a small amount of air. The juveniles of Xu's flat scorpionfish have a total length of 59.6mm ± 6mm (average total weight 3.79g), and fast for one day.

[0018] Use MS-222 produced by Fluka to prepare MS-222 mother liquor: Dissolve MS-222 in distilled water according to the ratio of MS-222: distilled water 1g: 10mL, then the density of the mother liquor is 0.1g / mL. Calculated according to the concentration of 100mg / L, 1.0g of MS-222 should be dissolved in 10L of seawater, that is, add 10mL of MS-222 mother liquor.

[0019] Use a net to fish the juvenile scorpionfish into the MS-222 seawater solution. Within 3 minutes, the juvenile fish lie on the bottom of the tan...

Embodiment 2

[0021] In September 2009, in the seed cultivation workshop of Yantai Baijia Aquatic Products Co., Ltd. located in Huangshanguan Town, Longkou City, Yantai, seawater with a water temperature of 22°C, a salinity of 29-30, a pH of 8.0-8.2, and a DO>6mg / L was used , with a 200L PVC tank as a container, seawater volume of 100L, a small amount of air. The juveniles of Xu's flat scorpionfish have a total length of 95.0mm ± 7mm (average total weight 13.79g), and fast for one day.

[0022] Use MS-222 produced by Fluka to prepare MS-222 mother liquor: Dissolve MS-222 in distilled water according to the ratio of MS-222: distilled water 1g: 10mL, then the density of the mother liquor is 0.1g / mL. Calculated according to the concentration of 110mg / L, 11.0g of MS-222 should be dissolved in 100L of seawater, that is, 110mL of MS-222 mother liquor should be added.

[0023] Use a net to fish the juvenile Scorpene scorpionfish into the MS-222 seawater solution. Within 3 minutes, the juvenile fi...

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PUM

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Abstract

The invention relates to technology of relieving stress response of fish, in particular to a method for relieving stress response in the manual operations of juvenile fish of sebastes schlegeli hilgendorf. The method is characterized by firstly stopping feeding the juvenile fish of sebastes schlegeli hilgendorf for a day to ensure the food in the intestines and stomach of the fish to be emptied; computing the dose of the required isotopic ethyl aminobenzoate alkane sulfonate (MS-222) according to the concentration of 90-110mg / L and dissolving the isotopic ethyl aminobenzoate alkane sulfonate into seawater and stirring to fully dissolve the isotopic ethyl aminobenzoate alkane sulfonate; and then spooning the juvenile fish of sebastes schlegeli hilgendorf into the MS-222 seawater solution, carrying out various test operations when the fish lies at the bottom of a water tank three minutes later and moving the juvenile fish of sebastes schlegeli hilgendorf into common seawater after the operations are completed. The optimum range of the concentration of the seawater solution is 100mg / L. Obviously, the method brings great convenience for such manual operations as transportation, individual marking, immunization injection, physiological experiment and the like, is characterized in that the juvenile fish does not contract diseases, and is fast and safe.

Description

technical field [0001] The invention relates to a technique for alleviating the stress response of fish, in particular to a method for alleviating the stress response in the manual operation of juvenile fish of the flat scorpionfish. Background technique [0002] Sebastes schlegeli Hilgendorf (Sebastes schlegeli Hilgendorf), commonly known as blackhead and black fish, is an offshore reef-loving demersal fish, distributed in the western North Pacific Ocean, in my country's Bohai Sea, Yellow Sea and East China Sea, especially in the Yellow Sea and Bohai Sea. Xu's flat scorpionfish is large in size, delicate in meat quality, high in taste, wide in seed sources, strong in disease resistance, low temperature resistant, and fast in growth. However, this fish is lively by nature and is highly sensitive to external stimuli; it struggles and jumps strongly after leaving the water, and there are sharp spines on the dorsal fin, anal fin, and gill cover, which are very easy to stab each...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘洪军关健官曙光高翔刘梦侠王其翔
Owner MARICULTURE INST OF SHANDONG PROVINCE
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