Method for judging salinity-tolerance potential of poplar with leaf epidermal cell death rate

A leaf epidermis and mortality technology, applied in material inspection products, color/spectral property measurement, biological testing, etc., can solve the problems of environmental factors, high molecular identification cost, long growth period of poplar trees, etc. Inexpensive, time- and labor-saving, speed-up effect

Inactive Publication Date: 2010-06-09
TIANJIN CITY AGRI BIO TECH RES CENT
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Problems solved by technology

However, the growth period of poplar is long, and it takes a long time to evaluate the growth shape, phenological traits, growth period, etc., w

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  • Method for judging salinity-tolerance potential of poplar with leaf epidermal cell death rate
  • Method for judging salinity-tolerance potential of poplar with leaf epidermal cell death rate
  • Method for judging salinity-tolerance potential of poplar with leaf epidermal cell death rate

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Embodiment Construction

[0020] Aiming at the problems of long time, many materials and low work efficiency in identifying the salt-tolerant potential of plants by traditional techniques, the inventors have carried out a lot of research work, and finally established a new method for quickly identifying the salt-tolerant potential of poplar by using the leaf epidermis cell death technology. method. Based on the ratio of dead cells in the leaf epidermis to the observed cells, the salt-tolerant potential of different genotypes can be quickly and accurately determined, so as to screen excellent genotypes with high salt-tolerant potential and eliminate a large number of genotypes in a short time. Useless materials, and then bred new varieties of poplar with good salt tolerance.

[0021] The present invention aims to provide a scientific and efficient salt-tolerant rapid identification method, through which a large number of salt-intolerant genotypes are eliminated, the combination of salt-tolerant genes an...

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Abstract

The invention relates to a method for judging salinity-tolerance potential of poplar with leaf epidermal cell death rate; the leaf epidermal cell of the poplar carries out plasmolysis under a high-salinity environment, the cells with different salinity-tolerance capacity have different plasmolysis degree, and the cells with high-degree plasmolysis can not be restored and die; natural red of dye stuff is used as indicator, the leaf epidermal cells of the poplar are dyed, different coloring of living cells and dead cells can judge the genetype of the poplars with different salinity-tolerance capacity; the leaf epidermal cell is treated in high-salinity solution for a certain time, the death rate of the leaf epidermal cells can rapidly and accurately reflect the salinity-tolerance capacity of the genetype; by utilizing the judging technique of the death rate of the leaf epidermal cell, the salinity-tolerance potential of the genetype can be accurately judged only for 20-30min, the labors and time are saved, the working efficiency is high, thereby having very wide application prospect.

Description

technical field [0001] The invention belongs to the plant detection of biotechnology, and is a method for identifying the salt-tolerant potential of poplar by leaf epidermis cell death rate. Background technique [0002] With the growth of the world's population and the improvement of people's living standards, land and fresh water resources are becoming more and more scarce, and land salinization has become one of the increasingly serious environmental problems. The development and utilization of vast medium-to-severe saline-alkali land can not only prevent further aggravation of soil salinization, but also expand the planting area of ​​farmland, and solve the contradiction between population increase and arable land decrease. Poplars are planted in saline-alkali, arid, barren and desert areas where there are few forests and trees. They can quickly restore vegetation, reduce wind and sand hazards, lower groundwater levels, protect farmland, reduce surface evaporation, incre...

Claims

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Application Information

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IPC IPC(8): G01N33/48G01N21/25
Inventor 郏艳红刘仲齐郝志愚王珠薛俊金凤媚聂莉莉张越宋建周祥明
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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