Method for preparing exorphins from soybean protein isolate by fractional hydrolysis

A technology of soybean protein isolate and step-by-step hydrolysis is applied in the field of preparation of exorphins, which can solve the problems of high cost and easy generation of harmful substances, and achieve the effects of low price, simple preparation method and cost reduction.

Inactive Publication Date: 2010-07-07
HARBIN UNIV OF COMMERCE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing exorphin by hydrolyzing soybean protein isolate in steps to solve the problems of high cost and easy production of harmful substances in the existing exorphin preparation method

Method used

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  • Method for preparing exorphins from soybean protein isolate by fractional hydrolysis
  • Method for preparing exorphins from soybean protein isolate by fractional hydrolysis
  • Method for preparing exorphins from soybean protein isolate by fractional hydrolysis

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specific Embodiment approach 1

[0013] Specific Embodiment 1: In this embodiment, the method for preparing exorphins by hydrolyzing soybean protein isolate step by step is carried out according to the following steps: 1. Put soybean protein isolate into pepsin for hydrolysis, the hydrolysis temperature is 36~38°C, and the pH is 1.8 ~2.2, the hydrolysis time is 2~6h, and the dosage of pepsin per gram of soybean protein isolate is 3500~4500U; 2. Put it into trypsin, acid protease, alkaline protease or neutral protease after pepsin hydrolysis Hydrolysis, the hydrolysis time is 2~6h, that is, exorphins are obtained.

[0014] The soybean protein isolate in step 1 of this embodiment is food grade (moisture 6.7182%, protein (dry basis) 82.6052%), and can be purchased from Harbin Gaoke Soybean Food Co., Ltd.

[0015] The exorphin prepared in this embodiment is tested by high-performance liquid chromatography, and the detection conditions are: C18 chromatographic column, wavelength 220nm, column temperature 25°C, inj...

specific Embodiment approach 2

[0018] Embodiment 2: This embodiment differs from Embodiment 1 in that the hydrolysis temperature in step 1 is 37° C., the pH is 2, the hydrolysis time is 5 hours, and the amount of pepsin per gram of soybean protein isolate is 4000 U. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0019]Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the conditions of trypsin hydrolysis in step two are: the hydrolysis temperature is 58~62°C, the pH is 7.5~8.5, and in every gram of soybean protein isolate The dosage of trypsin is 7000~9000U; the acid protease hydrolysis condition is: hydrolysis temperature is 42~48℃, pH is 2.5~3.5, the dosage of acid protease in each gram of soybean protein isolate is 5000~7000U; the alkaline protease hydrolysis condition is : The hydrolysis temperature is 51~57℃, the pH is 7.5~8.5, the amount of alkaline protease per gram of soybean protein isolate is 5000~7000U; the hydrolysis conditions of neutral protease are: the hydrolysis temperature is 52~58℃, the pH is 6.5~ 7.5, the dosage of neutral protease per gram of soybean protein isolate is 5000~7000U. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention provides a method for preparing exorphins from soybean protein isolate by fractional hydrolysis, which relates to a method for preparing exorphins. The invention solves the problem that the traditional exorphins preparing method has high preparing cost and is easy to produce harmful substances in the preparing process. The method 1: after being hydrolyzed, the pepsin is hydrolyzed by trypsin, acid protease, alkaline protease or neutral protease; the method 2, after being hydrolyzed, the trypsin is hydrolyzed by pepsin, acid protease, alkaline protease or neutral protease; the method 3, after being hydrolyzed, the alkaline protease is hydrolyzed by pepsin, trypsin, acid protease or neutral protease; the method 4, after being hydrolyzed, the neutral protease is hydrolyzed by pepsin, trypsin, acid protease or alkaline protease; and the method 5, after being hydrolyzed, the acid protease is hydrolyzed by pepsin, trypsin, alkaline protease or neutral protease. The invention has low cost, and does not produce harmful substances in the preparing process.

Description

technical field [0001] The present invention relates to a process for the preparation of exorphins. Background technique [0002] Exorphins are a class of biologically active peptides with morphine-like activity from outside the human body. They have positive physiological effects on the human body, such as analgesia, regulating human emotions, breathing, pulse, body temperature, etc. The preparation method of exorphins mainly contains two types: one, after extracting its own inherent natural active peptides from the organisms in nature, then further separation to obtain exorphins; two, synthetic methods to prepare exorphins, including Chemical synthesis, enzyme synthesis and recombinant DNA technology synthesis. Among them, the method of isolating exorphins from natural organisms, because the exorphins content in the organisms is very low, the extraction is difficult and the extraction cost is high, and a large amount of extraction will cause the shrinkage of species resou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02
Inventor 石彦国孙冰玉胡春林林宇红
Owner HARBIN UNIV OF COMMERCE
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