Immunogen composition containing polypeptide derived from cytomegalovirus and use method thereof

An immunogen and composition technology, applied in the field of vaccines, can solve problems such as virus contamination

Inactive Publication Date: 2010-07-21
VECTORITE BIOMEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of virus or virus-infected cells as an antigen source has the potential risk of virus contamination and infection; while the use of full-length herpes virus genes or proteins as an antigen source can induce immune suppression or immune tolerance Potential risks of (immune tolerance)

Method used

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  • Immunogen composition containing polypeptide derived from cytomegalovirus and use method thereof
  • Immunogen composition containing polypeptide derived from cytomegalovirus and use method thereof
  • Immunogen composition containing polypeptide derived from cytomegalovirus and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1 Efficacy Analysis of Stimulating Peripheral Blood Mononuclear Cells (PBMC) with Polypeptide Libraries Derived from Different CMV Proteins or Their Combinations---Using CFSE Staining Method

[0079] The purpose of this example is to use polypeptide libraries derived from different CMV proteins and combinations thereof to stimulate peripheral blood mononuclear cells, and use CFSE staining to analyze the effects of polypeptide libraries derived from different CMV proteins and combinations thereof on peripheral blood mononuclear cells The effect of the proliferative activity of the cells to understand the effect of different combinations on stimulating the immune response.

[0080] CFSE-labeled peripheral blood mononuclear cells (PBMC) were divided into the following groups according to the methods described in "Experimental Materials and Methods" and cultured by stimulating peripheral blood mononuclear cells with different polypeptide library combinations It last...

Embodiment 2

[0098] Example 2 Efficacy Analysis of Stimulating Peripheral Blood Mononuclear Cells (PBMCs) with Polypeptide Libraries or Combinations Derived from Different CMV Proteins---using ELISpot Analysis Method

[0099] The purpose of this example is to use polypeptide libraries derived from different CMV proteins and combinations thereof to stimulate peripheral blood mononuclear cells, and use ELISpot to analyze the effects of polypeptide libraries derived from different CMV proteins and combinations thereof on the cellular function of PBMCs.

[0100] Freshly isolated peripheral blood mononuclear cells from different donors were obtained according to the methods described in "Experimental Materials and Methods", and the PBMCs were stimulated with different polypeptide combinations in the following groups, and cultured and stimulated for 20 Hours, and IFN-γ analysis was performed using the ELISpot assay described in "Experimental Materials and Methods".

[0101] (1) CMV pentadecapept...

Embodiment 3

[0110] Example 3 Analysis of the proliferative effect of stimulating PBMC cells from various ethnic groups with the polypeptide library or combination derived from CMV protein

[0111] The purpose of this example is to utilize different CMV protein-derived polypeptide libraries and combinations thereof to stimulate PBMCs, and to analyze the effects of different CMV protein-derived polypeptide libraries and combinations thereof on CD4 in PBMCs + and CD8 + Effects on the proliferation of the population of cells.

[0112] CFSE-labeled PBMCs were represented by (1) pentadecapeptides of HCMVA pp65, denoted by "pp65"; (2) a mixture of pentadecapeptides with both HCMVA pp65 and HCMVA IE-1 Library (mixed pool), which includes HCMVA pp65 polypeptide library and HCMVA IE-1 polypeptide library, expressed as "pp65 / IE-1"; (3) has HCMVA pp65, HCMVA IE-1, HCMVA VPAP, HCMVA p100, HCMVT VGLB The mixed library of the five 15-peptide libraries is represented by "5pepmix"; (4) the non-specific ...

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PUM

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Abstract

The invention provides an immunogen composition containing polypeptide derived from cytomegalovirus (CMV) and a use method thereof. The composition comprises (a) at least one of CMV pp65 and polypeptide derived from CMV IE-1 polypeptide; and (b) polypeptide selected from groups formed by polypeptides derived from CMV VGLB, CMV VPAP and CMV p100 polypeptides, and the combination thereof. The immunogen composition of the invention can effectively activate CMV resistant immunoreaction after confirmation. Live virus, cells infected by CMV, and full-length CMV cells are not used in a polypeptide library so that no infection, mmunosuppression action, immunologic tolerance and the like are caused.

Description

technical field [0001] The present invention relates to an anti-herpes virus (herpesvirus) vaccine, in particular to an immunogen composition for producing anti-herpes virus immune effector cells (immune effector cells). The present invention also relates to the use of the aforementioned immunogen composition and its method for activating immune cells, belonging to the field of vaccines. Background technique [0002] Herpes viruses belong to the herpes virus family (Herpes viridae) virus, currently known to have eight viruses that can cause human diseases, these viruses are collectively referred to as human herpes virus (human herpes virus, HHV), including (1) human herpes virus-1 (human herpesvirus-1, HHV-1), also known as herpes simplex virus-1 (herpessimplexvirus-1, HSV-7); (2) human herpesvirus-2 (HHV-2), also known as herpes simplex virus-2 ( HSV-2); (3) Human herpes virus-3 (HHV-3), namely varicella zoster virus (Varicella ZosterVirus, VZV), can cause chickenpox and s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/245A61K39/39A61P31/20C12N5/08
Inventor 梁莹黄玉儒杨馥鸿
Owner VECTORITE BIOMEDICA
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