Immunogen composition containing polypeptide derived from cytomegalovirus and use method thereof
An immunogen and composition technology, applied in the field of vaccines, can solve problems such as virus contamination
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Examples
Embodiment 1
[0078] Example 1 Efficacy Analysis of Stimulating Peripheral Blood Mononuclear Cells (PBMC) with Polypeptide Libraries Derived from Different CMV Proteins or Their Combinations---Using CFSE Staining Method
[0079] The purpose of this example is to use polypeptide libraries derived from different CMV proteins and combinations thereof to stimulate peripheral blood mononuclear cells, and use CFSE staining to analyze the effects of polypeptide libraries derived from different CMV proteins and combinations thereof on peripheral blood mononuclear cells The effect of the proliferative activity of the cells to understand the effect of different combinations on stimulating the immune response.
[0080] CFSE-labeled peripheral blood mononuclear cells (PBMC) were divided into the following groups according to the methods described in "Experimental Materials and Methods" and cultured by stimulating peripheral blood mononuclear cells with different polypeptide library combinations It last...
Embodiment 2
[0098] Example 2 Efficacy Analysis of Stimulating Peripheral Blood Mononuclear Cells (PBMCs) with Polypeptide Libraries or Combinations Derived from Different CMV Proteins---using ELISpot Analysis Method
[0099] The purpose of this example is to use polypeptide libraries derived from different CMV proteins and combinations thereof to stimulate peripheral blood mononuclear cells, and use ELISpot to analyze the effects of polypeptide libraries derived from different CMV proteins and combinations thereof on the cellular function of PBMCs.
[0100] Freshly isolated peripheral blood mononuclear cells from different donors were obtained according to the methods described in "Experimental Materials and Methods", and the PBMCs were stimulated with different polypeptide combinations in the following groups, and cultured and stimulated for 20 Hours, and IFN-γ analysis was performed using the ELISpot assay described in "Experimental Materials and Methods".
[0101] (1) CMV pentadecapept...
Embodiment 3
[0110] Example 3 Analysis of the proliferative effect of stimulating PBMC cells from various ethnic groups with the polypeptide library or combination derived from CMV protein
[0111] The purpose of this example is to utilize different CMV protein-derived polypeptide libraries and combinations thereof to stimulate PBMCs, and to analyze the effects of different CMV protein-derived polypeptide libraries and combinations thereof on CD4 in PBMCs + and CD8 + Effects on the proliferation of the population of cells.
[0112] CFSE-labeled PBMCs were represented by (1) pentadecapeptides of HCMVA pp65, denoted by "pp65"; (2) a mixture of pentadecapeptides with both HCMVA pp65 and HCMVA IE-1 Library (mixed pool), which includes HCMVA pp65 polypeptide library and HCMVA IE-1 polypeptide library, expressed as "pp65 / IE-1"; (3) has HCMVA pp65, HCMVA IE-1, HCMVA VPAP, HCMVA p100, HCMVT VGLB The mixed library of the five 15-peptide libraries is represented by "5pepmix"; (4) the non-specific ...
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Description
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Application Information
- IPC
- A61K39/245; A61K39/39; A61P31/20; C12N5/08
- Inventors
- 梁莹; 黄玉儒