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Urinary polyamine detection kit

A urine polyamine and detection strip technology, applied in the field of urine polyamine detection kits, can solve the problems of high price, inability to meet tumor treatment and detection methods, etc., and achieves long storage time, no safety problems, and low technical operation requirements. Effect

Inactive Publication Date: 2010-07-21
孙晓平
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because most of the instruments used are imported and expensive, the chemiluminescence method is widely used in some large and medium-sized hospitals in China, but it is rarely used in some grassroots hospitals, and the reagents are traditional luminescent agents such as Lulinol and its derivatives. Lord, none of these can meet the needs of domestic development and popularization of tumor treatment and detection methods

Method used

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Effect test

Embodiment 1

[0035] Embodiment 1 urine polyamine detection kit of the present invention

[0036] The urinary polyamine detection kit of the present invention comprises the following parts: disposable detection strips (50 pieces), disposable sampler (50 pieces), disposable sampler (50 pieces), portable luminescent detector (1 piece), Instructions (1 copy).

Embodiment 2

[0037] The preparation of embodiment 2 detection strips

[0038] (1) Labeled standard antigen

[0039] Polyamine standards were purchased from Sigma (St. Louis, MO) (the ratio of polyamine components was putrescine:spermidine:spermine=8:1:1).

[0040] 0.5 μM polyamine and 2 μM isoluminolisothiocyanate (ILITC) were mixed and dissolved in 2 mL buffer (10 mM phosphate buffer, pH 7.3), sonicated for 1 minute, and stored in the dark for 20 minutes , by column chromatography (PD-10 desalting column, Amersham Pharmacia Biotech, Buckinghamshire, U.K.), eluted with 10mM Tris-HCl, 0.1mM EDTA to remove free ILITC, and recover the labeled polyamine antigen;

[0041] (2) Preparation of polyamine antibody

[0042] Take 600 μl (100 μg) of the labeled polyamine antigen and add it into Fluorine’s adjuvant at a volume ratio of 1:1, and phacoemulsify in an ice bath; select 3 points on each side of the back spine of the Japanese white rabbit, and inject 200 μl of polyamine and The mixture of e...

Embodiment 3

[0052] Embodiment 3 Portable Luminescence Detector

[0053] The portable luminescence detector in the urinary polyamine detection kit of the present invention is a weak luminescence measuring instrument developed by the Institute of Biophysics, Chinese Academy of Sciences (Chinese Patent No. ZL96241369.0, whose trade name is weak chemiluminescence and bioluminescence measuring instrument, model: KA-08) has been improved. In addition to the display screen and operation panel, a mechanical device (including a fishing hook) for operating the detection strip, a luminescent detection device, a signal processing device and a USB interface connected to the computer are added.

[0054] Such as figure 1 As shown, compared with the prior art (Chinese Patent No. ZL96241369.0), the improved part of the present invention includes:

[0055] 1. A mechanical device 18 for operating the test strip is added, which has the functions of ejecting and retracting the test strip support plate, displ...

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Abstract

The invention relates to a urinary polyamine detection kit, which comprises a detection strip, a luminescent detection instrument, a sampling device and a sample injector, wherein the detection strip comprises polyacrylamide gel, polyamine standard product, marked polyamine antigen, solid-phase antibody resin, luminescent agent, micro electrode and a plurality of plastic grooves. The invention also relates to a preparation method of the detection strip and an application of the urinary polyamine detection kit. The urinary polyamine detection kit can detect polyamine tumor markers in the urine sample, has the advantages of high sensitivity, high specificity, simple and rapid operation method, and the like, and is particularly used for ordinary families and the grass-root units to perform the tumor early prediction, the evaluation of curative effect and recrudescency monitoring.

Description

technical field [0001] The invention relates to a detection kit, in particular to a urine polyamine detection kit. Background technique [0002] Tumor is a malignant disease that seriously threatens human life and health. Early diagnosis and treatment is one of the most effective means to fight cancer. Tumor markers are a class of substances produced by tumor tissue that exist in the tumor tissue itself or are secreted into blood or other body fluids, or are stimulated by tumor tissue and produced by human cells, and their content is significantly different from the normal reference value. Tumor markers play an increasingly important role in the early diagnosis of tumors. [0003] Polyamines, including putrescine, spermidine and spermine, are a class of small organic molecule tumor markers whose concentration in tumor cells is significantly different from that in normal cells. The rapid growth of tumors is greatly dependent on the higher polyamine concentration in tumor ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N21/76G01N33/531G01N33/574
Inventor 孙晓平
Owner 孙晓平
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