Experimental method for verifying early process of radiation bystander effect based on arabidopsis thaliana

Abstract

The invention discloses an experimental method for verifying an early process of radiation bystander effect based on arabidopsis thaliana. The method comprises the following steps: adopting model plant arabidopsis thaliana as a material; carrying out ionizing radiation to the arabidopsis thaliana; then carrying out cutting and grafting treatment; extracting the AtRAD54 gene of new arabidopsis thaliana seedling which is grafted to the root tip of the radiated arabidopsis thaliana; and taking the expression level of the AtRAD54 gene as a detection end point. The innovation of the method lies in overcoming the limitation that the separation and connection of a radiated tissue (organ) and a non-radiated tissue (organ) can not be realized in animal experiments by adopting the characteristics of being capable of being cut and grafted which are caused by totipotency of a plant cell and providing a novel method system for studying the early process of remote radiation signals at the individual level.

Description

technical field [0001] The invention relates to the fields of biology and radiation detection, in particular to an experimental method for verifying the early process of radiation bystander effects based on Amaranthus shepherd's purse. Background technique [0002] In the traditional sense, the principle of radiation effect is that the energy of ionizing radiation is deposited in the radiation target (such as DNA, protein, etc.), which induces radiation damage, and then produces subsequent biological effects. However, studies in recent years have found that radiation effects are not limited to cells directly irradiated, and unirradiated bystander cells also exhibit biological effects similar to those of irradiated cells, that is, radiation induced bystander effect. , RIBE). [0003] In the 16 years since the discovery of radiation bystander effects in 1992, the research system of radiation bystander effects has mainly been in vitro cells. The research system is mainly divi...

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Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of radiation side effects early process verification experiment method based on Amaranthia shepherd's purse, take plants as objects, utilize the totipotency of plant cells, in order to solve the research of radiation side effects, it is difficult to detect its radiation side effects at the living body level Signal transduction and mechanism issues

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