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Photosensitive vesicle containing glycolipid combination probe for detecting avian influenza virus and preparation method thereof

A bird flu virus and combined probe technology, applied in the field of preparation of photosensitive vesicles, can solve complex synthesis problems, difficult to promote and other problems, and achieve the effects of easy synthesis, short color change response time, and low cost

Inactive Publication Date: 2012-05-23
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Since natural glycolipids or natural glycoproteins have multiple sialic acid-galactose sugar heads, if the natural glycolipids are artificially synthesized according to the detection requirements, a large number of complex synthesis problems will be encountered, and it is difficult to promote

Method used

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  • Photosensitive vesicle containing glycolipid combination probe for detecting avian influenza virus and preparation method thereof
  • Photosensitive vesicle containing glycolipid combination probe for detecting avian influenza virus and preparation method thereof
  • Photosensitive vesicle containing glycolipid combination probe for detecting avian influenza virus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The monosaccharide head glycolipid recognition molecules, phospholipid monomers, and diacetylene lipid monomers are miscibly dissolved in acetone, wherein the monosaccharide head glycolipid recognition molecules and the linkages that form the diacetylene-phospholipid photosensitive vesicle bilayer structure The total mol ratio of the lipid monomer of acetylene and the phospholipid monomer is 1: 100, and the mol ratio of the lipid monomer of diacetylene and the phospholipid monomer is 4: 1; Acetone is removed by rotary evaporation, and deionized water is added to make the combined The total concentration of acetylene lipid monomers and phospholipid monomers in deionized water is 0.01mmol / L, so that the monosaccharide head glycolipids are embedded in the phospholipid regions of the diacetylene-phospholipid bilayer of photosensitive vesicles by hydrophobic force; At a temperature of 90°C, the probe was ultrasonically hydrated for 10 minutes, so that the deionized water was ...

Embodiment 2

[0063] The monosaccharide head glycolipid recognition molecules, phospholipid monomers, and diacetylene lipid monomers are miscibly dissolved in dichloromethane, wherein the monosaccharide head glycolipid recognition molecules form a diacetylene-phospholipid photosensitive vesicle bilayer structure The total mol ratio of the lipid monomer of diacetylene and phospholipid monomer is 1: 100, and the mol ratio of the lipid monomer of diacetylene and phospholipid monomer is 1: 1; Potassium dihydrogen phosphate-sodium hydroxide buffer solution of 6.0, so that the total concentration of the lipid monomer and phospholipid monomer of diacetylene in the potassium dihydrogen phosphate-sodium hydroxide buffer solution is 200mmol / L, so that the monosaccharide head sugar Lipids are embedded into the phospholipid region of the diacetylene-phospholipid bilayer of photosensitive vesicles by hydrophobic force; at a temperature of 30 ° C, the water bath is ultrasonically hydrated for 180 minutes ...

Embodiment 3

[0077] Monosaccharide head glycolipid recognition molecules, phospholipid monomers, and diacetylene lipid monomers are miscibly dissolved in chloroform, wherein the monosaccharide head glycolipid recognition molecules and the linkages that form the diacetylene-phospholipid photosensitive vesicle bilayer structure The total molar ratio of the lipid monomer of acetylene to the phospholipid monomer is 1:5, and the molar ratio of the lipid monomer of diacetylene to the phospholipid monomer is 3:2; the chloroform is removed by rotary evaporation, and the phosphate with a pH of 6.9 is added Buffer solution, so that the total concentration of diacetylenic lipid monomers and phospholipid monomers in phosphate buffer solution is 2mmol / L, so that monosaccharide head glycolipids are embedded into the diacetylene-phospholipid bimolecules of photosensitive vesicles by hydrophobic force In the phospholipid region of the layer; at a temperature of 90 ° C, the probe was ultrasonically hydrated...

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Abstract

The invention belongs to the field of polydiacetylene vesicle biosensors with glycolipid probes, and particularly relates to a photosensitive vesicle containing a glycolipid combination probe for detecting avian influenza virus and a preparation method thereof. Monosaccharide head glycolipid identification molecules serving as the combination probe with the function of identifying the avian influenza virus are embedded into a phospholipid area of a polydiacetylene-phospholipid bilayer of the photosensitive vesicle by using a hydrophobic force, and the internal water phase is wrapped therein by the polydiacetylene-phospholipid bilayer to form the photosensitive vesicle; the polydiacetylene-phospholipid bilayer is polymerized by a lipoid monomer of diacetylene and a phospholipid monomer; and the monosaccharide head glycolipids embedded into the phospholipid area are lactose glycolipid or glucose glycolipid and sialic acid glycolipid. After the two monosaccharide head glycolipids are assembled, the capacity of identifying the avian influenza virus is remarkably improved compared with the capacity of single functional sialic acid saccharide head or sialic acid-galactose natural saccharide head for detecting the avian influenza virus, and the color change response time of the photosensitive vesicle is more transient.

Description

technical field [0001] The invention belongs to the field of polydiacetylene (PCDA) vesicle biosensors with glycolipid probes, in particular to photosensitive vesicles containing glycolipid combination probes for detecting avian influenza viruses, and the sugar-containing vesicles for detecting avian influenza viruses Preparation of photosensitive vesicles with lipid combination probes. Background technique [0002] Avian influenza (Avian Influenza, AI) is an infection or disease syndrome in poultry caused by Orthomyxoviridae / Influenzavirus / Influenza virus type A, and has been identified as a Class A infectious disease by the International Office of Epizootics. Low Pathogenic Avian Influenza (LAPI) mainly manifests from asymptomatic infection to moderate to mild respiratory disease and decreased egg production, while High Pathogenic Avian Influenza (HAPI) mainly manifests Systemic infection with high morbidity and mortality. There are many serotypes of this virus, and its ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/04C08F38/02C12R1/93
Inventor 江龙邓洁丽
Owner INST OF CHEM CHINESE ACAD OF SCI