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Serratia proteamaculans as well as preparation method and application thereof

A kind of panserratia, aspect technology, applied in the application field of preventing and treating wheat all-eater disease

Inactive Publication Date: 2012-11-14
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of endophytic bacteria colonized in wheat for biological control can overcome the shortcomings of the above-mentioned traditional biological control. It is necessary to strengthen the screening of wheat endophytic bacteria that have a high control effect on diseases. In the study of soil-borne diseases, there is no report on the control of wheat take-all by Serratia metamorphosus

Method used

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  • Serratia proteamaculans as well as preparation method and application thereof
  • Serratia proteamaculans as well as preparation method and application thereof
  • Serratia proteamaculans as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Select healthy wheat from the fields in the Huanghuai wheat area, take fresh wheat roots, wash the surface with tap water, absorb the water with filter paper, disinfect the surface with 75% ethanol for 30 seconds, disinfect with 0.1% mercury liter for 10 minutes, rinse with sterile water for 3 times, and cut into small pieces. Add 6 mL of sterile phosphate buffer (pH 7.0) to a sterile mortar and grind thoroughly. Stand still for 10 minutes and take the supernatant for 10-fold, 50-fold, and 100-fold dilution. Take 200 μL of each dilution and apply KMB (20 g of peptone, 10.0 g of glycerol, KMB 2 HPO 4 1.5g, MgSO 4 ·7H 2 O 1.5g, agar 15g, distilled water 1000mL, pH 7.2) plates, cultured at 28°C for 1-2d. After the colony grew out, it was purified and stored at 4°C.

[0028] Inoculate the preserved bacterial strains in liquid KMB medium for expanded culture (28°C, 200rpm, 1d), collect the bacteria by centrifugation, adjust the concentration of the homogeneous suspension...

Embodiment 2

[0030] The difference between this example and Example 1 is that the culture medium used for separation is improved LB, and its formula is: tryptone (tryptone) 5g, yeast extract (yeast extract) 2.5g, NaCl 5g, agar powder 15g dissolved in 800ml deionized water, adjust the pH to 7.5, add deionized water to a total volume of 1L.

[0031] Isolation method of Serratia proteamaculans wg-2

[0032] Select healthy wheat from the fields in the Huanghuai wheat area, take fresh wheat roots, wash the surface with tap water, absorb the water with filter paper, disinfect the surface with 75% ethanol for 30 seconds, disinfect with 0.1% mercury liter for 10 minutes, rinse with sterile water for 3 times, and cut into small pieces. Add 6 mL of sterile phosphate buffer (pH 7.0) to a sterile mortar and grind thoroughly. Stand still for 10 minutes and take the supernatant for 10 times, 50 times, 100 times dilution, take 200 μL of each dilution and apply KMB (peptone 20g, glycerol 10.0g, K2HPO41.5...

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Abstract

The invention relates to Serratia proteamaculans with the function of controlling wheat take-all as well as a preparation method and application thereof. The Serratia proteamaculans wg-2 has a preservation number of CCTCC NO: M209042. The bacterial strain carries out biological control on the wheat take-all by adopting the following steps of: carrying out 200rpm expanding culture on the bacterialstrain Serratia proteamaculans wg-2 with the preservation number of CCTCC NO: M209042 in a KMB fluid medium at 28 DEG C and then preparing a bacterial suspension solution; soaking wheat seeds for 1h,sowing the treated wheat seeds into disease-infected soil and carrying out root-irrigating treatment by using a bacterial fermentation solution. The bacterial strain is endogenous Serratia proteamaculans bacteria of wheat, can carry out large-scale colonization in wheat Neixiang 128, has better prevention effect (89.5 percent) to the wheat take-all, stable effect and favorable social and economic values and is easy to popularize and apply.

Description

technical field [0001] The present invention relates to a Serratia deformans, and also relates to the preparation method of the strain and its application in the prevention and treatment of wheat take-all. Background technique [0002] Wheat take-all (also known as blackfoot) is a soil-borne disease caused by Gaeumannomyces graminisvar tritici infecting the roots of wheat. After the wheat is infected with the disease, the seedlings will be thin and weak, the tillers will be reduced, the rate of ear formation will be reduced, and the thousand-grain weight will be reduced. The light ones will yield 10% to 20%, and the severe ones will reduce the yield by more than 50%, or even cause a failure. Once the wheat field is infected with the disease, it spreads quickly. It usually takes only 3 years for the sporadic disease to occur in a large area, and it is difficult to completely eradicate the disease in the diseased field. [0003] At present, for wheat soil-borne diseases, ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/425
Inventor 王刚王淼刘瑛王俊芳张颖
Owner HENAN UNIVERSITY
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