Broad-spectrum pesticide residue immunity test strip and preparation method and application thereof

Abstract

The invention discloses a broad-spectrum pesticide residue immunity test strip, which adopts a nitrocellulose membrane containing monoclonal or polyclonal antibodies for identifying the specificity of different organic phosphorus and different synthetic pyrethroid insecticides. The invention also discloses a preparation method and an application of the test strip. The test strip has the advantages that the measurement speed is fast and no particular device is required.

Description

technical field The invention relates to a detection test paper for pesticide residues and its preparation method and application, in particular to an immunological detection test paper for detection of various organophosphorus and various pyrethroid pesticide residues, its preparation method and application. Background technique Pesticides are indispensable substances for the prevention and control of diseases, insects, and weeds in current agricultural production. They play an extremely important role in promoting agricultural production, but at the same time they bring serious environmental problems: ecological imbalance, decline in the quality of agricultural products, and human health. Health is at risk. At present, organophosphorus and pyrethroid pesticides have highly effective insecticidal effects and are the main force of phytochemical protection. With the growing call for environmental protection in recent years, the environmental problems caused by the use of org...

AI Technical Summary

Problems solved by technology

Pesticides are indispensable substances for the prevention and control of diseases, insects, and weeds in current agricultural production. They play an extremely important role in promoting agricultural production, but at the same time they bring serious environmental problems: ecological imbalance, decline in the quality of agricultural products, and human health. health at risk

At present, the analysis methods of organophosphorus and pyrethroid pesticide residues are mainly complex pretreatment process, coupled with high-efficiency HPLC or GC (with ECD), this detection method is relatively expensive and requires skilled operators

Immunoassay is a fast and sensitive method for trace detection. However, most of the pesticides are small molecular compounds, and the synthesis of immunogens is difficult. Because of the specificity of antibodies, most of the established immunoassay methods are only suitable for a single pesticide. The detection and analysis of residues limits the application of immunoassay methods. In addition, most current immunoassays use polystyrene microtiter plates as solid phase carriers. The detection results generally take 3-8 hours, and the detection process is complicated.

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