System displacement multiple gene magnification technology

Abstract

The invention relates to 'the system displacement multiple gene magnification technology', which is characterized in that series target genes to be measured are displaced by a preselected probe into the same report sequence with different series probes, and a monotube magnification reaction of the same report sequence indicates multiple target molecules. The preselected probe is a target gene specific ( / conservative) short sequence and is used as a detection magnification template, and a pair of adjacent probe area primers is arranged on the short template; the report sequence is a long-sequence nucleic acid single chain which is heterogenous with a target and is divided into left and right parts, and the 3' ends of the long-sequence nucleic acid single chain are preconnected with the probe area primers, and the report sequence combines a target gene template and extends. The displacement is started from the second round of cycle, the probe primers takes the extended report sequence as a template to synthesize a full-length report sequence including left and right probe sequences; and then a report system with a target probe is magnified by using the primers at both ends of the report sequence. The report sequences which are magnified by the report system and have different sizes or different codes are monitored to indicate the multiple target molecules indirectly. More than one set of report sequence can be alternated.

Description

Technical field: [0001] The invention belongs to the technical field of molecular biology and the field of molecular detection application, and relates to an indirect polymerase chain reaction (Polymerase Chain Reaction) in which target gene amplification is replaced by reporter sequence amplification and its detection application. Background technique: [0002] Polymerase Chain Reaction (Polymerase Chain Reaction, PCR) is an in vitro nucleic acid amplification technique developed in the middle and late 1980s. It can amplify the target gene or a certain DNA fragment to be studied to more than one million times in one or two hours in a test tube, so that the naked eye can directly observe and judge on the gel; it can be obtained from a hair, A drop of blood, or even a single cell, can amplify enough DNA for analysis, research and identification. A target gene that could only be cloned in the past weeks to months can now be completed in a few hours by PCR. [0003] Nucleic a...

AI Technical Summary

Problems solved by technology

[0009] In order to overcome the problems of conventional PCR, such as easy cross-contamination false positives and target gene degradation false negatives, it is not easy to carry out the limitations of "surface" detection of parallel amplification of multiple target genes.

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