Method for preparing test paper for rapidly determining dairy cows subclinical ketosis

Abstract

The invention discloses a method for preparing a test paper for rapidly determining dairy cows subclinical ketosis, which comprises the following steps of: adding boric acid, aminoacetic acid and ethylene diamine tetraacetic acid solution in Arabic gum dissolved in a hot-water bath, adjusting the pH value of the prepared solution to be 7.0, soaking the test paper for 20 minutes in the prepared solution while the prepared solution is hot, drying the test paper in a drying oven at 60 DEG C, and standing the test paper in the drying oven overnight; and dissolving ethylene glycol, sodium nitroprusside and absolute ethyl alcohol in the hot-water bath, soaking the test paper for 20 minutes in the prepared solution while the prepared solution is hot, taking the test paper out, blotting moisture in the test paper by using filter paper, drying the test paper in the drying oven at 60 DEG C, and standing the test paper in the drying oven overnight so as to obtain the test paper. The reagent strip takes the concentration of beta-hydroxybutyric acid in blood more than 1.2mmol/L as a positive criteria; and the production practice proves that the reagent strip has the advantages of low cost, simple and easy operation, quick response, high specificity and high sensitivity, can be used for various determined samples such as blood, urine, serum, plasma and milk, and has wide application scope.

Description

1. Technical field [0001] The invention relates to the field of development of animal disease detection reagents, in particular to a method for preparing test paper for rapid determination of cow subclinical ketosis. 2. Background technology [0002] With the continuous improvement of dairy cow production and the continuous strengthening of intensive feeding, the incidence of nutritional and metabolic diseases of dairy cows has increased year by year. Among them, ketosis is a common metabolic disease in high-yield dairy cows. It is mainly caused by carbohydrate and lipid metabolism. Derangement arises, usually within a month of postpartum. Surveys show that the incidence of dairy cow ketosis in my country accounts for 10%-30% of lactating cows, and the incidence of subclinical ketosis is even higher. According to research reports, the incidence of dairy cow subclinical ketosis reaches 59%. The incidence of subclinical ketosis is 10-20 times that of clinical ketosis, but it ...

AI Technical Summary

Problems solved by technology

But such reagents are expensive to test for ketosis in dairy cows

However, domestic attention to ketosis is not enough, the rapid detection method is single, and the prevention and treatment methods cannot keep up with it. It is a simple method for the initial diagnosis of subclinical ketosis in dairy cows, but its sensitivity and specificity are low, and there are few reports on the quantitative determination of milk ketone body content to detect subclinical ketosis

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