Method for rapidly identifying tomato yellow leaf curl virus and papaya leaf curl China virus infecting tomato

Abstract

The invention provides a method for rapidly identifying two similar viruses (a tomato yellow leaf curl virus and a papaya leaf curl China virus) on a tomato, belonging to the field of plant protection. The method comprises the following steps: according to the nucleotide sequences of the tomato yellow leaf curl virus and the papaya leaf curl China virus, designing three primers, i.e. PA_F362, TY F833 and PATY_R, wherein the PA _ F362 and the PATY_ R are combined to detect the papaya leaf curl China virus, and the TY _ F833 and PATY _ R are combined to detect the tomato yellow leaf curl virus;and extracting total DNA of samples, and carrying out PCR only once, thus realizing identifying the tomato yellow leaf curl virus and the papaya leaf curl China virus. The method of the invention overcomes the problems of the traditional methods in biology, serology, electron microscope observation and the like; and compared with the method for detecting a virus by PCP once, the method of the invention reduces cost and saves time, thus being a specific, sensitive, economical and convenient detection method.

Description

technical field [0001] The invention relates to a method for quickly identifying two viruses on tomato (tomato yellow leaf curl virus and Chinese papaya leaf curl virus), belonging to the field of plant protection. Background technique [0002] Tomato yellow leaf curl virus (Tomato yellow leaf curl virus, TYLCV) is a very serious virus on tomato, which belongs to the genus Begomovirus (Geminiviridae) in classification, and the transmission medium is smoke. Whitefly (Bemisiatabaci), the viral genome is a single-stranded circular DNA with a size of 2.7-2.8kb. After the virus infects tomatoes, the main symptoms are that the leaves become smaller, roll up, yellowing between leaf margins and veins, plant growth retardation, internodes become shorter, dwarf, and the upper part is clustered, which has a great impact on tomato yield and quality. , Severely diseased fields often result in lost production and harvest. In the 1990s, the disease caused a 95% loss of local tomato produ...

AI Technical Summary

Problems solved by technology

Because these two viruses have very similar characteristics in many aspects, traditional plant virus detection methods, such as electron microscope observation (similar in size and shape of virus particles), biological inoculation identification (similar symptoms, same mediator, long cycle), Serological detection (belonging to the same genus, high sequence homology, serological correlation) and other methods are difficult to distinguish

At present, molecular biology methods are used in the detection of these two viruses. It is necessary to obtain their sequences for homologous comparison before they can be distinguished. There is no method to distinguish these two viruses using PCR.

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