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Tissue culture method of ophiopogon planiscapus

A technology of tissue culture and rye winter, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of small number of introductions, slow ramets, limitations, etc., to improve the uniformity and stability of seedlings , the effect of increasing the speed of reproduction

Inactive Publication Date: 2010-10-27
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a new variety, the number of introduced rye winters is small, and the ramets are also slow, so the supply of seedlings is limited to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Obtaining sterile materials

[0029] Take the young buds that have germinated in spring, remove the wrapped leaves, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and then soak them in mercury with a volume concentration of 0.5‰ for 10 minutes. Rinse 4 times with sterile water, use sterile filter paper to blot the water on the surface of the buds, cut the buds into 0.5cm-long segments with axillary buds, and inoculate the segments with MS+6-BA1.0mg / L+ NAA0.1mg / L bud induction medium;

[0030] (2) Differentiation and proliferation of buds

[0031] After the segments were inoculated on the bud induction medium for 2 weeks, the axillary buds began to expand and green protrusions appeared. After 4 weeks, bud meristems could be seen. After culturing for another month, the small buds could grow to 1cm. Cut off the small buds and transfer them to Into the proliferation medi...

Embodiment 2

[0040] (1) Obtaining sterile materials

[0041] Take the young buds that have germinated in spring, remove the wrapped leaves, wash them with tap water for 2 hours, put them on an ultra-clean workbench, and soak them in ethanol with a mass concentration of 75% for 30 seconds, and mercury with a volume concentration of 1‰ for 15 minutes. Rinse 5 times with sterile water again, use sterile filter paper to blot the water on the surface of the buds, cut the buds into 1cm-long segments with axillary buds, and inoculate the segments with MS+6-BA5.0mg / L+NAA0 .5mg / L bud induction medium;

[0042] (2) Differentiation and proliferation of buds

[0043] After the segments were inoculated on the bud induction medium for 3 weeks, the axillary buds began to expand and green protrusions appeared. After 5 weeks, bud meristems could be seen. After 2 months of culture, the small buds could grow to 2cm. Cut off the small buds and transfer them to Into the proliferation medium comprising MS+6-B...

Embodiment 3

[0052] (1) Obtaining sterile materials

[0053] Take the germinated young buds, remove the wrapped leaves, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and mercury with a volume concentration of 1‰ for 30 minutes, and then Rinse 6 times with sterile water, use sterile filter paper to blot the water on the surface of the buds, cut the buds into 2cm-long segments with axillary buds, and inoculate the segments with MS+6-BA5.0mg / L+NAA0. 5mg / L bud induction medium;

[0054] (2) Differentiation and proliferation of buds

[0055]After the segments were inoculated on the bud induction medium for 4 weeks, the axillary buds began to expand and green protrusions appeared. After 6 weeks, bud meristems could be seen. After 4 months of culture, the small buds could grow to 1.6cm, and the small buds were cut off. Transfer to the proliferation medium comprising MS+6-BA3.0mg / L+NAA0.4mg / L bud...

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Abstract

The invention relates to a tissue culture method of ophiopogon planiscapus, comprising the steps of asepsis material acquisition, bud differentiation, bud multiplication, adventitious bud strong seedling culture, rooting culture, seedling adaptation, transplantation and the like. Compared with the prior art, the invention greatly improves the reproductive speed of the ophiopogon planiscapus, the uniformity of seedlings and the stability of the performance and can realize factory mass production of the seedlings.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a ryepogon tissue culture method. Background technique [0002] Ophiopogon japonicus is a plant of the genus Ophiopogon japonicus in the family Liliaceae. It is native to the subtropical regions of my country and grows in moist places under dense forests in mountains and valleys. The leaves are linear, the top is blunt or gradually pointed, the edge of the leaves is rough, and the leaves are purple-black, which has high ornamental value. It is a good flower mirror and ground cover material. However, as a new variety, the number of introduced rye winters is small, and the ramets are also slow, so the supply of seedlings is limited to a certain extent. Contents of the invention [0003] The object of the present invention is to provide a method for tissue culture of ryepogon that can improve the propagation speed and the uniformity of seedlings and improve the stability of chara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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