Method for rapidly identifying peanut high-oleic acid material
An identification method and high oleic acid technology are applied in the field of identification of peanut high oleic acid materials, and can solve the problems of lack of large-scale fatty acid determination equipment and technical conditions, limitation of research progress of peanut high oleic acid, and high cost of sample testing. Achieve the effect of high reliability of identification results, low identification cost and low cost
Active Publication Date: 2012-03-07
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology
At present, the standard method for determining the content of oleic acid in peanuts is gas chromatography, but the operation is complicated, the cost of sample testing is expensive, and the requirements for equipment are relatively high. General peanut breeding units are not equipped with gas chromatography, and do not have the equipment for large-scale fatty acid determination and technical conditions limit the research progress of peanut high oleic acid breeding
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Embodiment 1
[0023] The rapid identification method of peanut high oleic acid materials includes the following steps:
[0024] 1) Preparation of peanut samples to be tested: Take healthy and plump peanut seeds as the test material, take a single peanut seed and cut it from the middle. The embryo-free part is used for identification, and the embryo-containing part is reserved for planting next year; if identified For a single peanut, you can cut off 1 / 5 of the whole peanut seed at the distal end of the hypocotyl, mix it for testing, and reserve the embryonic part for planting next year;
[0025] Extraction of peanut oil: select 24 samples of different peanuts to be tested with a mass content of oleic acid between 35.91% and 82.37% (see Table 1 for details), respectively number the different peanuts to be tested, and take 24 peanuts to be tested. 0.5g into each corresponding 2mL centrifuge tube (that is, there are 24 centrifuge tubes, and each centrifuge tube contains a peanut sample to be tested...
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Abstract
The invention relates to a method for identifying a peanut high-oleic acid material, in particular to a method for rapidly and simply identifying a peanut high-oleic acid material. The method comprises the following steps of: extracting peanut oil from a batch of peanut materials with larger oleic acid mass content difference; respectively determining oleic acid mass content (with a gas chromatography) and a refractive index of peanut oil; establishing a linear relation and a standard curvilinear equation of the oleic acid mass content and the refractive index; adopting the oleic acid mass content which is larger than or equal to 70 percent as a standard for the peanut high-oleic acid material, i.e. the refractive index which is not more than 1.4667 at standard temperature (25 DEG C) can be used as a judging standard for the peanut high-oleic acid material; determining a refractive index of an unknown peanut material, if the refractive index of the unknown peanut material at the standard temperature (25 DEG C) is larger than or equal to 1.4667, the unknown peanut material belongs to the peanut high-oleic acid material; and carrying out conversion to obtain the oleic acid mass content by utilizing the equation. The invention has simple operation and low cost, and the reliability of an identifying result of the method provided by the invention is as high as more than 95 percent.
Description
Technical field [0001] The invention relates to a method for identifying peanut high oleic acid materials. Background technique [0002] Peanut high oleic acid breeding is an important direction of peanut genetic improvement research. High oleic peanuts have the dual effects of improving the nutritional value of peanuts and their products and extending the shelf life. Oleic acid is considered to be the preferred fatty acid for balancing the contradiction between oil nutrition and chemical stability. At present, the standard method for determining the content of oleic acid in peanuts is gas chromatography, but the operation is complicated, the sample test cost is expensive, and the equipment requirements are high. The general flower breeding unit is not equipped with a gas chromatograph and does not have equipment for large-scale fatty acid determination. And technical conditions limit the research progress of peanut high oleic acid breeding. Summary of the invention [0003] The...
Claims
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IPC IPC(8): G01N21/41
Inventor 雷永廖伯寿姜慧芳王圣玉黄家权晏立英
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI