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Molecular marker of gene for regulating gelatinization temperature of rice and application thereof

A technology of gelatinization temperature and rice, applied in biochemical equipment and methods, DNA/RNA fragments, microbial determination/inspection, etc., can solve the problem of few molecular markers

Inactive Publication Date: 2010-11-10
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far there are few molecular markers that can be directly applied to GT breeding. If the genetic variation of each gene that controls rice GT can be transformed into operable molecular markers, combined with MAS and applied to rice quality breeding, it will be effectively promoted. Improvement of rice quality

Method used

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  • Molecular marker of gene for regulating gelatinization temperature of rice and application thereof
  • Molecular marker of gene for regulating gelatinization temperature of rice and application thereof
  • Molecular marker of gene for regulating gelatinization temperature of rice and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the application of SSII-3 molecular marker

[0042] The 15 varieties of rice were obtained from the Rice Research Institute of the Chinese Academy of Agricultural Sciences. The rice alkaline digestion value of 15 varieties of rice was detected by the method provided by the "Rice Quality Determination Standard of the Ministry of Agriculture of the People's Republic of China". After clustering, based on the SSII-3 gene (GENBANK ACCESSION NO.GQ150983), 15 rice varieties can be divided into 3 genotypes (see figure 1 -a), type I (composed of type I-a and type I-b) has a higher alkali digestion value (from 4.62 to 7.00, with an average value of 6.49), and has a lower gelatinization temperature; type II has a higher alkali digestion value Low (from 1.28 to 1.39, the average is 1.36), with a higher gelatinization temperature. The rice gelatinization temperature and genotype based on SSII-3 gene of 15 rice varieties are shown in Table 2.

[0043] Table 2 Rice ge...

Embodiment 2

[0048] Embodiment 2, the design and application of Wx molecular marker

[0049] After clustering, based on the Wx gene (GENBANK ACCESSION NO.GQ151053), 15 rice varieties can be divided into three genotypes (see figure 2 -a). Type ii is glutinous rice among cultivars. Under the same genotype background of SSII-3, the varieties of type ii have lower average alkali digestion value and higher gelatinization temperature than those of type i (composed of types i-a and type i-b). The rice gelatinization temperature and genotypes based on SSII-3 and Wx genes of different rice varieties are shown in Table 3.

[0050] Table 3 Rice gelatinization temperature of rice varieties based on SSII-3 and Wx genotypes

[0051]

[0052] Genomic DNA of rice was used as a template, PCR amplification was carried out with marker WxM1 (a primer pair composed of sequence 5 and sequence 6), the PCR amplification product was electrophoresed and sequenced to verify the electrophoresis result, and a 25...

Embodiment 3

[0055] Embodiment 3, the design and application of SBE3 molecular marker

[0056] After clustering, based on the SBE3 gene (GENBANK ACCESSION NO.GQ150913), 15 rice varieties can be divided into two genotypes (see image 3 -a). Type i includes 9 varieties including Nipponbare, type ii includes 6 varieties including 93-11. Under the same genotype background of SSII-3, the average alkali digestion value and gelatinization temperature of type ii varieties were slightly lower than those of type i varieties. The rice gelatinization temperature and genotypes based on SSII-3 and SBE3 genes of different rice varieties are shown in Table 4.

[0057] Table 4 Rice gelatinization temperature of rice varieties based on SSII-33 and SBE3 genotypes

[0058]

[0059] Genomic DNA of rice was used as a template, PCR amplification was performed with the marker SBE3M1, the PCR amplification product was subjected to electrophoresis and sequenced to verify the electrophoresis results, a 238bp D...

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Abstract

The invention discloses a molecular marker of a gene for regulating gelatinization temperature of rice and application thereof. The invention provides a method for identifying the rice with different gelatinization temperature in an assisted manner, comprising the following steps: taking the genome DNA of the rice to be tested as a template and carrying out PCR amplification by an SSII-3M1 primerpair; determining the genetype of the rice to be tested as type I if obtaining the 90bp DNA fragment; determining the genetype of the rice to be tested as type II if obtaining the 81bp DNA fragment; the alkali digestion value of the rice with genetype being type II being lower than that of the rice with genetype being type I; and forming the SSII-3M1 primer pair by the DNA shown in the sequence 1in the sequence table and the DNA shown in the sequence 2 in the sequence table. The invention has important theoretical value and practice significance in rice quality improvement, has direct application value in purposefully improving the rice quality by utilizing the genetic engineering technology or using the molecular marker to assist breeding and has certain theoretical value in studying biosynthesis mechanism of plant starch.

Description

technical field [0001] The invention relates to a rice gelatinization temperature regulation gene molecular marker and application thereof. Background technique [0002] Cultivating high-yield and high-quality new rice varieties has been the goal pursued by breeders for a long time. After decades of hard work, especially the use of hybrid rice technology, my country has achieved universally recognized achievements in rice production. However, in the past, how to solve people's food and clothing problems has always been the first priority, so the focus of rice breeding work is mostly concentrated on the cultivation of new high-yield varieties of rice, resulting in a relatively lagging behind in the breeding of high-quality rice, especially some high-yield varieties. The quality of hybrid rice generally deviates. [0003] One of the main reasons for the slow progress in rice quality improvement is its genetic complexity and the limitations of traditional research methods. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 李家洋顾铭洪钱前田志喜刘巧泉严长杰刘贵富王永红
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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