Unlock instant, AI-driven research and patent intelligence for your innovation.
Method for preparing culture solution by using waste water in chondroitin production as fermentation nitrogen source
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of culture medium and chondroitin, which is applied in the field of comprehensive utilization of wastewater resources, can solve problems such as increasing the load of sewage treatment equipment, easy corruption of sewage, and excessive COD values, achieving remarkable ecological and economic benefits and reducing sewage treatment The effect of increasing load and solubility
Inactive Publication Date: 2012-05-02
许累峰
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Since the waste water is mainly composed of organic matter, the COD value seriously exceeds the standard, and the ordinary activated sludge method is difficult to treat. The sewage is easily corrupted, deteriorated, smelly, pollutes the water body, and damages the environment.
The current treatment method for this wastewater is to directly enter the sewage treatment link, which not only increases the load on the sewage treatment device, but also causes a waste of resources
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0015] 1. Add 100kg of fresh sheep cartilage to 50kg of water for cooking, add compound animal protease for enzymolysis, and obtain 120kg of enzymolysis solution. The enzymolysis solution absorbs chondroitin through resin, and produces 110kg of waste water. The solid content of waste water is 18wt%, and the nitrogen content is 1.25wt%. The content is about 7.8wt%, and the waste water is filtered.
[0016] 2. According to waste water solid content 19.8g / L, sucrose 70g / L, MgSO 4 6g / L, KH 2 PO 4 The content of 5g / L was used to prepare the fermentation broth.
[0017] Add 800kg of water and MgSO to 110kg of waste water 4 8kg, KH 2 PO 4 5kg, fully dissolved, mixed evenly, sterilized at 121°C for 20min, added 70kg of sterilized sucrose, added sterile water to 1000L, adjusted the pH of the medium to 6.8, and obtained the fermentation broth.
[0018] Add the culture solution in step 2 into a fermenter to ferment hyaluronic acid, and culture it at 37° C. and pH 6.8 for 30 hou...
Embodiment 2
[0020] 1. Add 150kg of fresh shark cartilage to 75kg of water for cooking and enzymolysis, add alcohol to precipitate chondroitin in the enzymolysis solution, and distill alcohol to produce 120kg of waste water with a solid content of 22wt%.
[0021] 2. According to waste water solid content 30g / L, soluble starch 50g / L, MgSO 4 10g / L, KH 2 PO 4 4g / L to prepare fermentation broth,
[0022] Add 500kg of water and MgSO to 120kg of waste water 4 8.8kg, KH 2 PO 4 3.52kg, fully dissolved, mixed evenly, sterilized at 121°C for 20 minutes, added 44kg of sterilized soluble starch, added sterile water to 880L, adjusted the pH of the culture solution to 6.8, and obtained a fermentation culture solution.
[0023] Add the fermentation culture liquid in step 2 into a fermenter to ferment hyaluronic acid, control the temperature at 37°C, stir at a speed of 220r / min, ventilate at 1.2vvm, adjust the pH to 6.8 with NaOH, and cultivate for 28 hours to obtain a mature culture liquid. The...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to the field of water reuse, in particular to a comprehensive utilization method for preparing a fermentation culture solution by using waste water in chondroitin production, which comprises the following steps: preparing chondroitin from cartilage through an enzymolysis-alcohol extraction method; recovering by alcohol to obtain the waste water with 2-12wt% of amino acid; mixing and dissolving the waste water with MgSO4, KH2PO4 and water, then sterilizing at high temperature; adding sterilized carbon source at a certain ratio; and evenly mixing, and adjusting a pH value to 6-8 to obtain the culture solution, wherein the carbon source is cane sugar or soluble starch, the waste water solid content in the culture solution is 10-30g / L, the content of the MgSO4 is 6-10g / L, the content of KH2PO4 is 4-5g / L, and the content of the carbon source is 50-70g / L. The invention has the beneficial effects of effectively utilizing the amino acid, trace elements and growth factorsin the waste water, reducing sewage treatment load, providing nitrogen source at a high quality for fermentation production and reducing the fermentation liquor cost.
Description
technical field [0001] The invention relates to the field of comprehensive utilization of waste water resources, in particular to a comprehensive utilization method for preparing fermentation culture liquid from waste water in the production process of chondroitin. Background technique [0002] Fermentation nitrogen sources commonly used in microbial fermentation production include beef extract, peptone, yeast powder, etc. Beef extract, yeast powder, peptone, etc. are rich in amino acids and nucleotides, and contain more vitamins and other growth factors needed for microbial growth. They are high-quality nitrogen sources for fermentation, but the price is relatively high, and the cost of fermentation medium larger. Therefore, choosing a low-cost, nutrient-rich nitrogen source to reduce production costs and improve the quality of fermentation medium has become one of the problems to be solved in the field of fermentation. [0003] At present, the production of chondroitin m...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More