Fermentation method for producing polyhydroxyalkanoate
A technology of polyhydroxyalkanoate and fermentation method, which is applied in the direction of fermentation, etc., which can solve the problems of affecting yield, high price, and toxicity of production bacteria, and achieve the effects of improving product purity, reducing production cost, and shortening fermentation time
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Embodiment 1
[0020] The first step: extract polyhydroxyalkanoate producing bacteria,
[0021] Add the activated sludge containing cornstarch wastewater into the SBR reactor, control the temperature at 45°C, the pH value at 6, the hydrolysis and acidification time is 26 hours, and stir for 10 minutes, then let it stand naturally, and wait for the sediment to settle completely to get Bacterial suspension;
[0022] Step 2: Obtain high-density bacteria
[0023] The cell suspension containing polyhydroxyalkanoate-producing bacteria is obtained through seed cultivation to obtain activated cells, and the obtained cells are used as seeds for large-scale cultivation to obtain high-density polyhydroxyalkanoate-producing bacteria .
[0024] The third step: using polyhydroxyalkanoate-producing bacteria to produce polyhydroxyalkanoate monomers and polymers,
[0025] The above-mentioned clear solution was added into a fermenter with cornstarch at a ratio of 15%, the temperature was controlled at 45°C...
Embodiment 2
[0029] The first step: extract polyhydroxyalkanoate producing bacteria,
[0030] Add the activated sludge containing cornstarch wastewater into the SBR reactor, control the temperature at 55°C, control the pH value at 5, hydrolyze and acidify the time for 22 hours, and stir for 10 minutes, then let it stand naturally until all the precipitates are precipitated, and get Bacterial suspension;
[0031] Step 2: Obtain high-density bacteria
[0032] The cell suspension containing polyhydroxyalkanoate-producing bacteria is obtained through seed cultivation to obtain activated cells, and the obtained cells are used as seeds for large-scale cultivation to obtain high-density polyhydroxyalkanoate-producing bacteria .
[0033] The third step: using polyhydroxyalkanoate-producing bacteria to produce polyhydroxyalkanoate monomers and polymers,
[0034] The above-mentioned clear solution was added into a fermenter with cornstarch in a proportion of 15%, the temperature was controlled at...
Embodiment 3
[0038] The first step: extract polyhydroxyalkanoate producing bacteria,
[0039] Add the activated sludge containing cornstarch wastewater into the SBR reactor, control the temperature at 48°C, and control the pH value at 5.5. The hydrolysis and acidification time is 24 hours, and stirred for 10 minutes, and then placed naturally until all the precipitates are precipitated, and the obtained Bacterial suspension;
[0040] Step 2: Obtain high-density bacteria
[0041] The cell suspension containing polyhydroxyalkanoate-producing bacteria is obtained through seed cultivation to obtain activated cells, and the obtained cells are used as seeds for large-scale cultivation to obtain high-density polyhydroxyalkanoate-producing bacteria .
[0042] The third step: using polyhydroxyalkanoate-producing bacteria to produce polyhydroxyalkanoate monomers and polymers,
[0043] The above-mentioned clear solution was added into a fermenter with cornstarch in a proportion of 15%, the tempera...
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