Multiple PCR identification method of salmonella serogroup A, B, C1, C2 or D

Abstract

The invention relates to a multiple PCR identification method of salmonella serogroups A, B, C1, C2 or D, belonging to the technical field of food safety detection. The method comprises the following steps: step 1: respectively designing specific amplification primer pairs according to a base sequence such as 5 nucleic acids as shown in SEQ ID NO:1-5; and step 2: detecting a sample by utilizing the specific amplification primer pairs obtained in the step 1 and adopting a conventional PCR method, and determining the type of the salmonella serogroup in the sample. The determined type of the salmonella serogroup in the sample is concretely as follows: the gel electrophoresis detection is carried out, wherein if 350bp and 466bp bands exist, the result shows that the serogroup A exists; if 177bp bands exist, the result shows that the serogroup B exists; if 623bp bands exist, the result shows that the serogroup C1 exists; if 540bp bands exist, the result shows that the serogroup C2 exists; and if 466bp bands exist, the result shows that the serogroup D exists. The method of the invention can be used for quickly and accurately identifying the salmonella of the serogroups A, B, C1, C2 or D, and avoids the defects of complicated operation, time and labor consumption and high cost of the existing method.

Description

technical field [0001] The invention relates to a multiplex PCR identification method in the technical field of food safety detection, in particular to a multiplex PCR identification method for Salmonella serogroups A, B, C1, C2 and D. Background technique [0002] Salmonella is one of the most important food-borne pathogens, which can be transmitted through food (especially animal food), and can cause human gastroenteritis, sepsis, dysentery and typhoid fever. Serotyping is the core of its identification research in the study of Salmonella epidemiology, and plays an important role in monitoring disease outbreaks, transmission trends and disease control. At present, there are more than 2,500 serotypes of Salmonella isolated in the world, and there are about 300 serotypes in my country, but the serotypes related to human diseases are mainly concentrated in A~E serogroups, and the pathogenic Salmonella in A~D groups account for the majority. to 70%. [0003] In practice, the ...

AI Technical Summary

Problems solved by technology

Since the antigenic structure of Salmonella is polymorphic, the gene sequences in the corresponding rfb gene clusters are also different in sequence, deletion, and mutation among the various Salmonella serotypes, so it is not easy to design a multiplex assay for PCR identification. Primers, so most of the detection methods are identified by detection chips, which makes the identification cost too high

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