Method for rapidly testing nitrite in fresh milk

Abstract

The invention provides a method for rapidly testing nitrite in fresh milk. The method comprises the following steps: adding 3-6ml of milk sample in a colorimetric tube, then adding 1-2ml of 20% tartaric acid solution and 1-2ml of 0.5% sulfanilic acid solution in turn, shaking evenly, adding 1-2ml of 0.3% N(1-naphty1)-ethylenediamine dihydrochloride solution, wherein the lower dosage of 20% tartaric acid solution is 1ml, the lower dosage of 0.5% sulfanilic acid solution is 1ml, and the lower dosage of 0.3% N(1-naphty1)-ethylenediamine dihydrochloride solution is 1ml; and observing after fully shaking, wherein if different shades of pink appears in the colorimetric tube, the sample is proved to contain nitrite. The size of the selected colorimetric tube with a stopper is 15-20ml. The lower limit detection value of the method is 0.1mu g / ml; and the effects of the method are that the dosage is small, the preservation time is long and the detection sensitivity is high. The reagent kit prepared by the method is more convenient to use.

Description

technical field

[0001] The invention relates to the quality detection of fresh milk, especially the determination of ingredients containing nitrite. In order to ensure its food safety, the inspection box made by this method is more convenient to use. Background technique

[0002] With the improvement of living standards, milk has become an indispensable part of people's lives. Milk is rich in protein, fat, carbohydrates, trace elements and vitamins, etc. Eating milk can enhance physical fitness and improve intelligence, so it is deeply loved by consumers. However, in order to make huge profits, it is common for lawbreakers to add nitrite to fresh milk. Nitrite directly acts on human blood vessels, causing peripheral blood vessels to dilate, and can form methemoglobin with hemoglobin in the blood, leading to clinical symptoms such as dizziness, nausea, vomiting, and even blood pressure drop or shock. In order to protect the health of consumers, it is necessary to strictly t...

Images