Three-layer electrostatic spinning ordered fiber nerve conduit and preparation and application thereof

Abstract

The invention relates to a three-layer electrostatic spinning ordered fiber nerve conduit and preparation and application thereof. The conduit comprises an inner layer, a middle layer and an outer layer, wherein the inner layer is a micron fibrous membrane, the middle layer is a polymer degradable reticulate conduit, and the outer layer is a submicron fibrous membrane with a three-dimensional reticulate structure. The preparation comprises the following steps of: (1) dissolving a PHBV in chloroform / acetone solution to prepare mixed solution; (2) performing electrostatic spinning on the mixed solution to prepare a fibrous membrane; (3) taking the fibrous membrane from a winding bobbin, and winding into an inner pipe; (4) weaving polymer degradable fibers into the reticulate conduit, and sleeving on the inner pipe; and (5) fixing a core rod sleeved with the inner pipe and the reticulate conduit on a high-speed winding and collecting device, and performing the electrostatic spinning on the mixed solution. The conduit has double functions of the nerve conduit and a tissue engineering support, and is favorable for the attachment of cells and the growth and the expansion of nerves alonga fiber parallel direction; and the preparation method has the advantages of simpleness, no environmental pollution and suitability for industrialized production.

Description

technical field

[0001] The invention belongs to the field of nerve guide and its preparation and application, in particular to a three-layer electrospinning ordered fiber nerve guide and its preparation and application. Background technique

[0002] Since Hueter first proposed the comprehensive repair of peripheral nerve injuries by the adventitia in 1873, humans have made continuous efforts for the repair of peripheral nerves, and the repair methods have also been continuously improved. The main methods are:

[0003] (1) The direct end-to-end suture method directly sutures the outer membranes of the two stumped nerves, and completes the repair by utilizing the regeneration of the nerves. This method is suitable for the repair of short-distance stumped nerves. This method cannot be implemented when the nerve stump distance is long.

[0004] (2) Autologous nerve transplantation is currently the most commonly used clinical method and is considered the "gold standard" for long...

Examples

Embodiment 1

[0037] (1) Dissolving the PHBV in a volume ratio=4:1 chloroform / acetone solution and stirring evenly to obtain a PHBV / chloroform / acetone solution with a mass percent of PHBV of 7%;

[0038] (2) Carry out electrospinning of PHBV / chloroform / acetone solution, the solution propulsion speed is 1.5mL / h, the applied voltage value is 7kv, the receiving distance is 15cm, and it is received by high-speed winding drum to obtain a fiber film with good orientation ;

[0039] (3) After the electrospinning is finished, remove an ordered fiber film from the winding drum, and wind it on a mandrel to form an inner tube with fibers arranged in the axial direction, and the diameter of the mandrel is about 2mm;

[0040] (4) Weave polyglycolic acid (PGA) fibers into a 3mm mesh circular tube, put it on the inner tube, and stretch it slightly to fix it;

[0041] (5) Fix the mandrel covered with the inner tube and the intermediate tube on the high-speed winding collection device, and carry out the el...

Embodiment 2

[0044] (1) Dissolving PHBV in a volume ratio=7:1 chloroform / acetone solution, stirring evenly to obtain a PHBV / chloroform / acetone solution with a mass percent of PHBV of 6%;

[0045] (2) Carry out electrospinning of PHBV / chloroform / acetone solution, the solution propulsion speed is 1mL / h, the applied voltage value is 10kv, the receiving distance is 13cm, and the high-speed winding drum is used to receive, and a fiber film with good orientation is obtained;

[0046] (3) After the electrospinning is finished, remove an ordered fiber film from the winding drum and wind it on a mandrel to form an inner tube in which the fibers are arranged in the axial direction, and the diameter of the mandrel is about 1mm;

[0047] (4) Weave polycaprolactone (PCL) fibers into a 2mm mesh circular tube, put it on the inner tube, and stretch it slightly to fix it;

[0048] (5) Fix the mandrel covered with the inner tube and the intermediate tube on the high-speed winding collection device, and carr...

Embodiment 3

[0051] (1) Dissolving PHBV in a volume ratio=5:1 chloroform / acetone solution, stirring evenly to obtain a PHBV / chloroform / acetone solution with a mass percentage of PHBV of 5%;

[0052] (2) Carry out electrospinning of PHBV / chloroform / acetone solution, the solution propulsion speed is 0.5mL / h, the applied voltage value is 15kv, the receiving distance is 17cm, and it is received by high-speed winding drum to obtain a fiber film with good orientation ;

[0053] (3) After the electrospinning is finished, remove an ordered fiber film from the winding drum, and wind it on a mandrel to form an inner tube with fibers arranged in the axial direction, and the diameter of the mandrel is about 2.5mm;

[0054] (4) Weave polyglycolide lactide (PGLA) fibers into a 3mm mesh circular tube, put it on the inner tube, and stretch it slightly to fix it;

[0055] (5) Fix the mandrel covered with the inner tube and the intermediate tube on the high-speed winding collection device, and perform elec...

technical field

[0001] The invention belongs to the field of nerve guide and its preparation and application, in particular to a three-layer electrospinning ordered fiber nerve guide and its preparation and application. Background technique

[0002] Since Hueter first proposed the comprehensive repair of peripheral nerve injuries by the adventitia in 1873, humans have made continuous efforts for the repair of peripheral nerves, and the repair methods have also been continuously improved. The main methods are:

[0003] (1) The direct end-to-end suture method directly sutures the outer membranes of the two stumped nerves, and completes the repair by utilizing the regeneration of the nerves. This method is suitable for the repair of short-distance stumped nerves. This method cannot be implemented when the nerve stump distance is long.

[0004] (2) Autologous nerve transplantation is currently the most commonly used clinical method and is considered the "gold standard" for long...

Examples

Embodiment 1

[0037] (1) Dissolving the PHBV in a volume ratio=4:1 chloroform / acetone solution and stirring evenly to obtain a PHBV / chloroform / acetone solution with a mass percent of PHBV of 7%;

[0038] (2) Carry out electrospinning of PHBV / chloroform / acetone solution, the solution propulsion speed is 1.5mL / h, the applied voltage value is 7kv, the receiving distance is 15cm, and it is received by high-speed winding drum to obtain a fiber film with good orientation ;

[0039] (3) After the electrospinning is finished, remove an ordered fiber film from the winding drum, and wind it on a mandrel to form an inner tube with fibers arranged in the axial direction, and the diameter of the mandrel is about 2mm;

[0040] (4) Weave polyglycolic acid (PGA) fibers into a 3mm mesh circular tube, put it on the inner tube, and stretch it slightly to fix it;

[0041] (5) Fix the mandrel covered with the inner tube and the intermediate tube on the high-speed winding collection device, and carry out the el...

Embodiment 2

[0044] (1) Dissolving PHBV in a volume ratio=7:1 chloroform / acetone solution, stirring evenly to obtain a PHBV / chloroform / acetone solution with a mass percent of PHBV of 6%;

[0045] (2) Carry out electrospinning of PHBV / chloroform / acetone solution, the solution propulsion speed is 1mL / h, the applied voltage value is 10kv, the receiving distance is 13cm, and the high-speed winding drum is used to receive, and a fiber film with good orientation is obtained;

[0046] (3) After the electrospinning is finished, remove an ordered fiber film from the winding drum and wind it on a mandrel to form an inner tube in which the fibers are arranged in the axial direction, and the diameter of the mandrel is about 1mm;

[0047] (4) Weave polycaprolactone (PCL) fibers into a 2mm mesh circular tube, put it on the inner tube, and stretch it slightly to fix it;

[0048] (5) Fix the mandrel covered with the inner tube and the intermediate tube on the high-speed winding collection device, and carr...

Embodiment 3

[0051] (1) Dissolving PHBV in a volume ratio=5:1 chloroform / acetone solution, stirring evenly to obtain a PHBV / chloroform / acetone solution with a mass percentage of PHBV of 5%;

[0052] (2) Carry out electrospinning of PHBV / chloroform / acetone solution, the solution propulsion speed is 0.5mL / h, the applied voltage value is 15kv, the receiving distance is 17cm, and it is received by high-speed winding drum to obtain a fiber film with good orientation ;

[0053] (3) After the electrospinning is finished, remove an ordered fiber film from the winding drum, and wind it on a mandrel to form an inner tube with fibers arranged in the axial direction, and the diameter of the mandrel is about 2.5mm;

[0054] (4) Weave polyglycolide lactide (PGLA) fibers into a 3mm mesh circular tube, put it on the inner tube, and stretch it slightly to fix it;

[0055] (5) Fix the mandrel covered with the inner tube and the intermediate tube on the high-speed winding collection device, and perform elec...

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