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Composite of hepatitis B virus core antigen and heat shock protein and preparation method and application thereof

A technology of hepatitis B virus and heat shock protein, which is applied in the field of protein complexes, can solve the problems that have not yet been reported on the research of hepatitis B therapeutic vaccine, achieve good development and application prospects, simple preparation method, and the effect of inhibiting replication

Active Publication Date: 2010-12-15
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, vaccines based on antigenic peptide-HSP complexes are mostly constructed using HSP70, HSP90 and GP96, and are mostly used in the research of tumor treatment. There is no research report on the construction of hepatitis B therapeutic vaccines using GRP170

Method used

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  • Composite of hepatitis B virus core antigen and heat shock protein and preparation method and application thereof
  • Composite of hepatitis B virus core antigen and heat shock protein and preparation method and application thereof
  • Composite of hepatitis B virus core antigen and heat shock protein and preparation method and application thereof

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Embodiment Construction

[0021] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0022] 1. Preparation of HBcAg-GRP170 complex

[0023] 1. Preparation of HBcAg

[0024] (1) Cloning of HBcAg gene

[0025] According to the HBcAg gene sequence with GenBank accession number NC 003977.1, the following primers were designed and synthesized to amplify the full-length HBcAg cDNA with restriction sites at both ends: F1: 5’-cg gaattc ggcatggacatcgacccttat-3' (SEQ ID No.3), the underlined part is the EcoR I restriction site; R1: 5'ccg ctcgag agttccccacccttatgagtc-3' (SEQ ID No.4), ...

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Abstract

The invention discloses a composite for hepatitis B virus core antigen and heat shock protein and a preparation method and application thereof. The composite is combined by the hepatitis B virus core antigen and the glucose-regulated protein 170 through a non-covalent bond, can activate and recognize the specificity CTL of the hepatitis B virus core antigen, induces strong antiviral immunity without any adjuvant, and is not limited by MHC in same species application. The preparation method for the composite is simple, can be used for preparing hepatitis B therapeutic vaccines and has good development and application prospect in the field of hepatitis B immune therapy.

Description

technical field [0001] The invention relates to a protein complex, in particular to the complex of hepatitis B virus core antigen and heat shock protein, and also relates to the preparation method and application of the complex. Background technique [0002] Hepatitis B virus (HBV) is a hepatotropic DNA virus. Hepatitis B virus hepatitis B, referred to as hepatitis B, is caused by HBV, mainly through blood, body fluids and mother-to-child transmission, and has a chronic carrier state of infectious diseases. At present, there are more than 300 million chronic HBV infections in the world, about half of which are in my country. This kind of infection is easy to develop into chronic hepatitis and cirrhosis, and a small number of cases can be transformed into primary hepatocellular carcinoma. It is currently the ninth cause of death among human diseases announced by WHO. Therefore, seeking an ideal anti-HBV drug has always been an urgent research topic. At present, chronic HBV...

Claims

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Application Information

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IPC IPC(8): C07K19/00A61K39/29A61P1/16A61P31/20
Inventor 吴玉章杨曌
Owner ARMY MEDICAL UNIV
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