Swine fever negative and positive serum reference materials and preparation method thereof

A standard substance and positive serum technology, applied in biological testing, material inspection products, etc., can solve the problems of low virus proliferation titer, error, and increased difficulty

Inactive Publication Date: 2010-12-15
CHINA INST OF VETERINARY DRUG CONTROL
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  • Abstract
  • Description
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Problems solved by technology

However, since the cells infected by CSFV do not cause cell lesions, and the virus proliferation titer is low, it is more difficult for virus detection, product inspection, and scientific research, and the experimental results are easily affected by the experience, ability, experimental facilities and environment of personnel Therefore, it is urgent to use reference materials as objective and impartial scal...

Method used

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  • Swine fever negative and positive serum reference materials and preparation method thereof
  • Swine fever negative and positive serum reference materials and preparation method thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0058] 1. Preparation of Candidates

[0059] (1) Animal selection Select the pathogens of foot-and-mouth disease, pseudorabies, swine fever, porcine reproductive and respiratory syndrome, porcine parvovirus, bovine viral diarrhea / mucosal disease, and negative antibodies (the pathogens are detected by PCR or RT-PCR, and the antibodies Detect with ELISA method) pig as experimental animal;

[0060] (2) Antigen preparation Inject 1ml each of CSFV Shimen strain (CVCC AV1411), 03 strain (CVCC AV03) and 22 strains (CVCC AV22) intramuscularly into 3 pigs, and collect blood from the carotid artery when the pigs are dying, and use glass Beads are defibrillated and anticoagulated, and stored below -20°C;

[0061] Antigen test: according to the "Chinese Veterinary Pharmacopoeia", the results meet the requirements of pure test and safety test;

[0062] (3) Prepare a series of sera with different titers according to different immunization procedures:

[0063] 1) Negative serum preparation ...

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Abstract

The invention relates to swine fever negative and positive serum reference materials and a preparation method thereof. The swine fever negative and positive serum reference materials are prepared by using China-domesticated, separated and identified swine fever viruses to immunize negative swine and adopting processes of sampling blood, separating serum, irradiating, filtering, split charging, freezing-drying, melting sealing, inspecting and the like, and by final cooperative calibration and constant value preparation. The swine fever negative and positive serum reference materials prepared by the method has the advantages that: the preparation process is scientific, strict and complete; the specificity is high; the application range is wide; and the accuracy of the constant value is high.

Description

technical field [0001] The invention relates to a standard substance of swine fever negative serum positive serum and a preparation method thereof, and belongs to the technical field of standard substance technology and veterinary biological products. Background technique [0002] Classical Swine Fever (CSF) is a highly contagious and fatal swine infectious disease caused by Classical Swine Fever Virus (CSFV), which occurs in many countries and regions in the world. Industrial production has brought serious economic losses. The World Organization for Animal Health (OIE) lists swine fever as a statutory notifiable disease, and my country classifies it as a first-class animal infectious disease. Therefore, the research and control of swine fever has received extensive attention worldwide, and our country is no exception. However, since the cells infected by CSFV do not cause cell lesions, and the virus proliferation titer is low, it is more difficult for virus detection, pro...

Claims

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Application Information

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IPC IPC(8): G01N33/96
Inventor 戴志红关孚时王在时蒋卉李翠
Owner CHINA INST OF VETERINARY DRUG CONTROL
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