Swine fever negative and positive serum reference materials and preparation method thereof

Abstract

The invention relates to swine fever negative and positive serum reference materials and a preparation method thereof. The swine fever negative and positive serum reference materials are prepared by using China-domesticated, separated and identified swine fever viruses to immunize negative swine and adopting processes of sampling blood, separating serum, irradiating, filtering, split charging, freezing-drying, melting sealing, inspecting and the like, and by final cooperative calibration and constant value preparation. The swine fever negative and positive serum reference materials prepared by the method has the advantages that: the preparation process is scientific, strict and complete; the specificity is high; the application range is wide; and the accuracy of the constant value is high.

Description

technical field [0001] The invention relates to a standard substance of swine fever negative serum positive serum and a preparation method thereof, and belongs to the technical field of standard substance technology and veterinary biological products. Background technique [0002] Classical Swine Fever (CSF) is a highly contagious and fatal swine infectious disease caused by Classical Swine Fever Virus (CSFV), which occurs in many countries and regions in the world. Industrial production has brought serious economic losses. The World Organization for Animal Health (OIE) lists swine fever as a statutory notifiable disease, and my country classifies it as a first-class animal infectious disease. Therefore, the research and control of swine fever has received extensive attention worldwide, and our country is no exception. However, since the cells infected by CSFV do not cause cell lesions, and the virus proliferation titer is low, it is more difficult for virus detection, pro...

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Problems solved by technology

However, since the cells infected by CSFV do not cause cell lesions, and the virus proliferation titer is low, it is more difficult for virus detection, product inspection, and scientific research, and the experimental results are easily affected by the experience, ability, experimental facilities and environment of personnel Therefore, it is urgent to use reference materials as objective and impartial scales for product quality control, reference for scientific research and laboratory diagnosis, and for inter-laboratory capabilities. Comparison

However, the quality requirements of CSF negative serum and positive serum standard substances are high, and the preparation methods, inspection and calibration methods are strict, and the relevant preparation procedures and calibration methods have not been reported so far.

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