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Brain-targeting neurotrophic factor fusion polypeptide for preventing and treating Alzheimer's disease

A neurotrophic factor, fusion polypeptide technology, applied in the field of biomedicine, can solve problems such as easy to cause immune response, lack of central orientation, etc.

Active Publication Date: 2010-12-22
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently known vectors have significant limitations and disadvantages
For example: TFR lacks central orientation and can cause immune response; OX26 / 8D3 is easy to cause immune response; M6P receptor is only expressed in young mammals; RAP is easy to be cleared by the body's clearance mechanism

Method used

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  • Brain-targeting neurotrophic factor fusion polypeptide for preventing and treating Alzheimer's disease
  • Brain-targeting neurotrophic factor fusion polypeptide for preventing and treating Alzheimer's disease
  • Brain-targeting neurotrophic factor fusion polypeptide for preventing and treating Alzheimer's disease

Examples

Experimental program
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Embodiment

[0069] Example Preparation of the fusion polypeptide of the present invention

[0070] 1. Escherichia coli expression vector of neurotrophic factor active peptide-junction fragment (Ahx)-11 peptide (YGRKKRRQRRR) fusion gene and Escherichia coli directed multiple Copy the expression vector, express and purify in Escherichia coli.

[0071] 1.1 Screen the target neurotrophic factor and its possible active fragments in the human gene bank;

[0072] 1.2 Determine the sequence of the target neurotrophic factor and its possible active fragments;

[0073] 1.3 Construct the fusion gene E. coli expression vector of the target neurotrophic factor and its possible active fragment and -11 peptide;

[0074] 1.4 Construction of an E. coli-directed multi-copy expression vector for the active fragment of the target neurotrophic factor and the -connecting fragment (Ahx)-11 peptide.

[0075] 1.5 Expression and purification in Escherichia coli.

[0076] Express the fusion gene of target neuro...

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Abstract

The invention provides a brain-targeting neurotrophic factor fusion polypeptide for preventing and treating Alzheimer's disease, which is composed of a neurotrophic factor active peptide segment, 11 peptide and 6-aminohexanoyl (Ahx) in the middle of the neurotrophic factor active peptide segment and the 11 peptide. An amino acid sequence of the 11 peptide is YGRKKRRQRRR, and the neurotrophic factor active peptide segment comprises a brain-derived neurotrophic factor (BDNF), a nerve growth factor (NGF) or a ciliary neurotrophic factor (CNTF). The fusion polypeptide can penetrate a blood-brain barrier and can be used for preventing and treating Alzheimer's disease.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to neurotrophic factor polypeptide drugs that can pass through the blood-brain barrier and prevent and treat senile dementia. Background technique [0002] Alzheimer's disease is the most common neurodegenerative disease and a serious threat to human health. Its pathological feature is the formation of neurofibrillary tangles (neurofibrillary tangles) mainly composed of hyperphosphorylated microtubule-associated protein tau in a large number of neurons. , NFTs) and a large number of senile plaques (SP) formed by β-amyloid polypeptide (β-amyloid, Aβ) deposition and outside neurons, accompanied by a large number of cholinergic neurons and neuronal synapses Decrease, clinically manifested as progressive cognitive decline. [0003] At present, there are no therapeutic drugs and methods aimed at the pathological characteristics of senile dementia. The drugs approved by t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12N15/62C12N15/70A61K38/07A61K38/08A61K47/48A61P25/28A61K47/64
Inventor 王建枝
Owner HUAZHONG UNIV OF SCI & TECH
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